Protein Info for MIT1002_03030 in Alteromonas macleodii MIT1002

Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 521 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 23 to 515 (493 residues), 493.6 bits, see alignment E=3.3e-152 PF01225: Mur_ligase" amino acids 26 to 106 (81 residues), 44.2 bits, see alignment E=3.1e-15 PF08245: Mur_ligase_M" amino acids 118 to 343 (226 residues), 156.1 bits, see alignment E=1.8e-49 PF02875: Mur_ligase_C" amino acids 366 to 492 (127 residues), 109.5 bits, see alignment E=3.2e-35

Best Hits

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 92% identity to amc:MADE_03263)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (521 amino acids)

>MIT1002_03030 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase (Alteromonas macleodii MIT1002)
MVVSAFIQSIKALLAKFGVDAPDIRIEGLTLDSREVNTRLAFLAIKGHERDGRDFIPQAI
SLGARVILAQTEDSSAHGQMEMRDHSVIISLYELPQMLSALAAAFYDYPAQKMTTVAVTG
TNGKTSTVQLLTQLKDALGTRSASIGTLGSSVFDGDETQWSNTPAANTTPDAIRMQYLLA
DFVQSEVRHTAFEASSHALVQGRLGTVKTDIAVFTNLTRDHLDYHGTMEEYGKAKRLLLQ
QPGLKAVVLNANDDESKKWDGAADKSITRIWTGIDESGLDGSGESEYLREHNNPQSRHCF
ATNASYHAGGCSFELVSSWGSAAISLPLFGKFNISNVLSAIGTLLAEGERFDDVVNAVNA
ITPVPGRMEVFPVTGAANLVVDYAHTPDALEKVLLSAKFHTTGKVWCVFGCGGDRDKGKR
PLMGQAAEVGADAIVITTDNSRSEDPESIAEDIMAGLNNRELAVSIPDREQAIRHCLEHA
HESDIIIVAGKGHEDYQIIGDKKTNYNERVVVARLQQEYSK