Protein Info for MIT1002_03025 in Alteromonas macleodii MIT1002

Annotation: UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 372 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01133: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase" amino acids 3 to 362 (360 residues), 334.1 bits, see alignment E=4.9e-104 PF03033: Glyco_transf_28" amino acids 6 to 142 (137 residues), 155.3 bits, see alignment E=2.1e-49 PF13579: Glyco_trans_4_4" amino acids 21 to 170 (150 residues), 37.4 bits, see alignment E=6.6e-13 PF13439: Glyco_transf_4" amino acids 22 to 172 (151 residues), 31.6 bits, see alignment E=3.3e-11 PF04101: Glyco_tran_28_C" amino acids 197 to 345 (149 residues), 129.8 bits, see alignment E=2.1e-41

Best Hits

Swiss-Prot: 88% identical to MURG_ALTMD: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K02563, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [EC: 2.4.1.227] (inferred from 88% identity to amc:MADE_03258)

Predicted SEED Role

"UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)" in subsystem Peptidoglycan Biosynthesis (EC 2.4.1.227)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.227

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (372 amino acids)

>MIT1002_03025 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (Alteromonas macleodii MIT1002)
MAKRCLIMAGGTGGHVFPGLAVANALRKEGWDIHWLGTAERMEAQVVPKHDIPIHFIPVK
GLRGKGVTARLQGAVALVKSLFSARRIIKRLQPDIVVGFGGYASGPGGVAAKSLGIPVIV
HEQNAAAGMTNKLLSKFASRVLLGFDDAKEQFSGGATKVHTVGNPVRDEIWQVKPKSVDI
DADARENTTEHKQGLNLLVVGGSLGAQILNETVPETCGVLEGLSIKHQCGKGNSEGVIKA
YTSVGADMSKIDVSDFIDDMAAAYEWADFVVCRAGALTVSEVAAAGRAAIFVPLPFAVDD
HQTKNAQSLVKQNAALMIAQSVLKENLGQAVRQWLQHPEGCLKMGAVAKTCASTHATENV
VSHVKSVVGVGD