Protein Info for MIT1002_03024 in Alteromonas macleodii MIT1002

Annotation: UDP-N-acetylmuramate--L-alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 17 to 464 (448 residues), 579.9 bits, E=2.2e-178
• PF01225: Mur_ligase
  • amino acids 18 to 115 (98 residues), 97.6 bits, E=6.6e-32
• PF08245: Mur_ligase_M
  • amino acids 120 to 300 (181 residues), 103.8 bits, E=1.8e-33
• PF02875: Mur_ligase_C
  • amino acids 322 to 409 (88 residues), 55.1 bits, E=1.2e-18

Best Hits

Swiss-Prot: 69% identical to MURC_SHEB5: UDP-N-acetylmuramate--L-alanine ligase (murC) from Shewanella baltica (strain OS155 / ATCC BAA-1091)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 96% identity to amc:MADE_03257)

MetaCyc: 62% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (6/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (10/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (11/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (11/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (10/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Protein Sequence (481 amino acids)

>MIT1002_03024 UDP-N-acetylmuramate--L-alanine ligase (Alteromonas macleodii MIT1002)
MVFKTPFESPQMRRVKNIFFIGIGGAGMGGIAEVLLNEGYAIAGSDIQEGPMTQRLSELG
ADVKIGHRAENVEGMDVIVVSSAINEQNPEIIVAREKRIPIIRRAEMLAELMRFRHGIAV
AGTHGKTTTTSLISTIFAEAEYDPTFVIGGLLNSAGTNARLGKSRYLIAEADESDASFLH
LQPMVSVVTNIEADHMDTYGGDFSKVKDTFVDFLSNLPFYGQAIVCGDDANIQEIRPRIG
RSILTYGFESHNDYRAENVSLSFGKANFTVIRPEREPLDITLNLTGNHNVLNALASIAVA
TDEGVDDDAIIRALASFGGIGRRFEQLGTFTTSKGEVCLVDDYGHHPTEVRATIAAARAN
WPDKRLVMVYQPHRFTRTRDLYEDFVEVLSSVDALLLLDVYAASEQPIEGADSKALCRSI
RQRGQIEPVYVGDKDALPAILANIMQDGDIVMTQGAGNIGQIAKLLARHVLEPITLAKEG
E