Protein Info for MIT1002_02973 in Alteromonas macleodii MIT1002

Annotation: Aminomethyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00528: glycine cleavage system T protein
  • amino acids 4 to 354 (351 residues), 487 bits, E=1.6e-150
• PF01571: GCV_T
  • amino acids 7 to 254 (248 residues), 298.6 bits, E=3.4e-93
• PF08669: GCV_T_C
  • amino acids 280 to 352 (73 residues), 60 bits, E=1.7e-20

Best Hits

Swiss-Prot: 99% identical to GCST_ALTMD: Aminomethyltransferase (gcvT) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K00605, aminomethyltransferase [EC: 2.1.2.10] (inferred from 99% identity to amc:MADE_00861)

MetaCyc: 70% identical to aminomethyltransferase (Escherichia coli K-12 substr. MG1655)
Aminomethyltransferase. [EC: 1.4.1.27, 2.1.2.10]; 1.4.1.27 [EC: 1.4.1.27, 2.1.2.10]

Predicted SEED Role

"Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 2.1.2.10)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• glycine degradation (3/3 steps found)
• photorespiration I (7/9 steps found)
• folate transformations I (9/13 steps found)
• photorespiration III (6/9 steps found)
• photorespiration II (6/10 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Nitrogen metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.10

Use Curated BLAST to search for 1.4.1.27 or 2.1.2.10

Protein Sequence (359 amino acids)

>MIT1002_02973 Aminomethyltransferase (Alteromonas macleodii MIT1002)
MSSTTALHAKHLEAGAKMVDFFGWDMPINYGSQIEEHHAVRQDAGMFDVSHMTIVDVKGA
QAKAYLQYLLANDVAKLKDKGKALYSGMLNEEGGVVDDLIVYHFDETNYRLVVNSATREK
DMNWLMSKAEGFEVTITERPEFAMIAVQGPNAKEKAATLFSAAQKEAVAGMKPFFGVQAE
DLFIATTGYTGEAGYEIMVPAEQAESFWQALLDAGVKPCGLGARDTLRLEAGMNLYGQDM
DETVSPLAANMGWTITWEPADRDFVGRKALEAQREAGTDKLVGLVMTEKGVLRHGLKVKT
ESGEGVITSGTFSPTLGHSIAMARVPSDVGETVEVEMRKKWVTVKVVKPSFVRNGKSVL