Protein Info for MIT1002_02833 in Alteromonas macleodii MIT1002

Annotation: Malate synthase G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01345: malate synthase G
  • amino acids 4 to 727 (724 residues), 1128.8 bits, E=0
• PF20656: MS_N
  • amino acids 18 to 68 (51 residues), 37.8 bits, 2.2e-13
• PF20658: MSG_insertion
  • amino acids 158 to 233 (76 residues), 99.9 bits, E=1.5e-32
• PF01274: MS_TIM-barrel
  • amino acids 343 to 572 (230 residues), 204 bits, E=4.1e-64
• PF20659: MS_C
  • amino acids 596 to 677 (82 residues), 85.1 bits, E=1.1e-27

Best Hits

Swiss-Prot: 58% identical to MASZ_RHOFA: Malate synthase G (glcB) from Rhodococcus fascians

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 94% identity to amc:MADE_01324)

Predicted SEED Role

"Malate synthase G (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• chitin deacetylation (3/4 steps found)
• glycolate and glyoxylate degradation II (1/2 steps found)
• superpathway of glycol metabolism and degradation (3/7 steps found)
• L-arabinose degradation IV (2/8 steps found)
• D-xylose degradation IV (1/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (1/14 steps found)
• superpathway of pentose and pentitol degradation (4/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Protein Sequence (731 amino acids)

>MIT1002_02833 Malate synthase G (Alteromonas macleodii MIT1002)
MQGYITRGRLQVAEQLDNFINEQALPGTGVDKDTFWQGAETLFEKFIPQNRALLEKREQL
QRAIDDYHKAGNPVNGNEYTDFLKSIGYLVEQPSQVSAQTQNVDAEIATMAGPQLVVPIN
NARYALNAVNARWGSLYDALYGTDVINNEGGAEAGKSYNPVRGAKVVAKAKDYLDSMIPL
AKGSHADVTEYKIESGKLAITLVDGDETTLANTEQWLGYQGDISAPSSLLFAHNGLHFEI
QIDPTHPIGESDAANVKDVLVEAALTTIMDCEDSVAAVDAEDKTLVYSNWLGLMKGSLSI
EMTKGGKQLVRAMHDDRSYQPSKHASQDKLKDGELVLSGRSLMFVRNVGHLMTNNAMLFD
GEEVPEGIMDGLVTSLIAKHDLLGNSKFKNSREGSIYIVKPKMHGPEEVAFSNALFGAIE
AIIDVPANTLKMGIMDEERRTSINLQACIAEATSRVVFINTGFLDRTGDEIHTSMLAGPF
AEKAALKSMAWIKAYETANVAVGLRCGLSGKAQIGKGMWPIPDEMQNMMTAKIGHPKAGA
NTAWVPSPTAATLHALHYHDVNVFDVQKSLDENFSGLADILTIPLLEDASTLTRESIKRE
IDNNVQGILGYVVRWVHQGVGCSKVPDINNVGLMEDRATLRISSQHIANWLEHGIVSVDQ
VKESLARMAVLVDEQNAGDPEYIPMAPDLDSSIGFLAASDLIFKGKEQPSGYTEPLLHAR
RREMKAKLAAS