Protein Info for MIT1002_02700 in Alteromonas macleodii MIT1002

Annotation: Riboflavin biosynthesis protein RibD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 PF00383: dCMP_cyt_deam_1" amino acids 8 to 111 (104 residues), 78.6 bits, see alignment E=4.3e-26 PF14437: MafB19-deam" amino acids 10 to 115 (106 residues), 43.8 bits, see alignment E=3.7e-15 TIGR00326: riboflavin biosynthesis protein RibD" amino acids 13 to 368 (356 residues), 383.7 bits, see alignment E=8.6e-119 TIGR00227: riboflavin-specific deaminase C-terminal domain" amino acids 157 to 371 (215 residues), 193 bits, see alignment E=4.9e-61 PF01872: RibD_C" amino acids 158 to 368 (211 residues), 167.3 bits, see alignment E=5.9e-53

Best Hits

KEGG orthology group: K11752, diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase [EC: 1.1.1.193 3.5.4.26] (inferred from 71% identity to alt:ambt_04355)

Predicted SEED Role

"Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193)" in subsystem Riboflavin, FMN and FAD metabolism (EC 1.1.1.193, EC 3.5.4.26)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.193 or 3.5.4.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (379 amino acids)

>MIT1002_02700 Riboflavin biosynthesis protein RibD (Alteromonas macleodii MIT1002)
MNHDTVKNDYFWMAKAIQLAQQGRFTTSPNPRVGCVIVDENNQLLGQGYHIQAGTPHAEV
HALRQASNARPQGAKGATAYVTLEPCSHFGRTPPCAVALVEAKVARVVIAMTDPNPNVCG
NGISILQEAGIEVVSEVMAAEAAALNPGFIKRMLTGRPFVRVKLGISLDGKIALQNGVSQ
WITGAEARRDVQQYRAQSCAVLTGSGTVQADNPSLLVREKEAQFEKYPLDHIRQPARVVV
DSKSKLSRDYTLFNDGNITFTATSQAHPDTATQHYLQLDEKDGKLNLHSLMSALGDKQYN
EVWVEAGPGLAGALLQEGLVDELICYQAPKLLGDKGVSMVNLPAFTSLNECISLSLIENR
QVGNDIKLIFRPDVSNTRG