Protein Info for MIT1002_02672 in Alteromonas macleodii MIT1002

Annotation: D-lactate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01565: FAD_binding_4
  • amino acids 41 to 168 (128 residues), 35 bits, E=1.1e-12
• PF09330: Lact-deh-memb
  • amino acids 271 to 561 (291 residues), 440.7 bits, E=2.8e-136

Best Hits

KEGG orthology group: K03777, D-lactate dehydrogenase [EC: 1.1.1.28] (inferred from 89% identity to amc:MADE_01443)

Predicted SEED Role

"D-Lactate dehydrogenase (EC 1.1.2.5)" in subsystem Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.5)

MetaCyc Pathways

• heterolactic fermentation (15/18 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• mixed acid fermentation (13/16 steps found)
• L-alanine degradation II (to D-lactate) (3/3 steps found)
• pyruvate fermentation to (R)-lactate (1/1 steps found)
• vancomycin resistance I (1/3 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (5/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (2/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.28, 1.1.2.5

Use Curated BLAST to search for 1.1.1.28 or 1.1.2.5

Protein Sequence (567 amino acids)

>MIT1002_02672 D-lactate dehydrogenase (Alteromonas macleodii MIT1002)
MQTTPIIDAFKRVLGESNLAVGTRQTEHYRKGWRSGEGEALAVLFPHTLMQYWEVLKVCV
EYNCIIIMQAAKTGLTEGSTPSGSDYDRPVVVINTLAMDDIVLVNDGTQVLSFPGATLFK
LEKALKPLGRAPHSVIGSSCLGASIVGGVANNSGGALVKRGPAYTELSLFAQVDKNGELK
LVNHLGIDLGDTPEEIIGNLSSGNFPKSLPETSMKASASDYEYIVRDVNADTPSRFNADP
TRLYEASGCAGKIAVFAVRLDTFEVPNKEQTFYIGTNDASVLTRIRRDILTQCKNVPEVG
EYLHREMFDIADVYGKDTIISIQRLGTDSLPKMFALKGRVDAVLNKLPLLPPYLSDRLMQ
FGSKFFSDQIPSSIRTYRDKYEHHLILKMSDGGIEEVKTLLAKLFNEEKLDGDFITCNEE
EASKAFLLRFAAAGAAVRYQTLHHKQVGDILALDIALRRNDDLWVENLPEHIAKHVEKSL
YYGHFFCHVFHQDYILKKGSDAKKVKADMLALLDSRGAKYPAEHNVGHLYEAESNLQKFY
KDLDPTNTFNPGIGKMEKHKRNCSCCL