Protein Info for MIT1002_02524 in Alteromonas macleodii MIT1002

Annotation: Amidophosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 507 TIGR01134: amidophosphoribosyltransferase" amino acids 2 to 464 (463 residues), 520.8 bits, see alignment E=1.9e-160 PF13230: GATase_4" amino acids 64 to 172 (109 residues), 24 bits, see alignment E=3.5e-09 PF13522: GATase_6" amino acids 64 to 200 (137 residues), 60.6 bits, see alignment E=3.3e-20 PF13537: GATase_7" amino acids 80 to 213 (134 residues), 47.6 bits, see alignment E=3.3e-16 PF00156: Pribosyltran" amino acids 279 to 392 (114 residues), 31.9 bits, see alignment E=1.6e-11

Best Hits

Swiss-Prot: 71% identical to PUR1_ECOLI: Amidophosphoribosyltransferase (purF) from Escherichia coli (strain K12)

KEGG orthology group: K00764, amidophosphoribosyltransferase [EC: 2.4.2.14] (inferred from 94% identity to alt:ambt_05085)

MetaCyc: 71% identical to amidophosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Amidophosphoribosyltransferase. [EC: 2.4.2.14]

Predicted SEED Role

"Amidophosphoribosyltransferase (EC 2.4.2.14)" in subsystem De Novo Purine Biosynthesis (EC 2.4.2.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (507 amino acids)

>MIT1002_02524 Amidophosphoribosyltransferase (Alteromonas macleodii MIT1002)
MCGIVGIVGKTPVAQSLYDGLTVIQHRGQDAAGIMTIDQNMFNLRKANGLVRDVFHTRHM
KRLSGNMGIGHVRYPTAGTSSSAEAQPFYVNSPFGIAFAHNGNLTNAHELQEEVFRIARR
HINTTSDSELLLNILAHELQQVAGLSVSAEHIFEVVTKVHKKIRGAYAVVAAIIGQGIVA
FRDPHGIRPLALGKRMSELGEEWMVASESVALDAVGFQFVRDVMPGEAVFITEEGQLHTR
QCAENPVTSPCIFEFVYFARPDSFIDGISVYASRVNMGRKLGEKIKREWADLDIDVVIPI
PETSMDVALQIANTLDLPYRQGFVKNRYIGRTFIMPGQTMRKKSVRRKLNAISSEFKGKS
VLLVDDSIVRGTTSGQIIEMARESGAKKVYFASAAPEIRFPNVYGIDMPSANELIAYGRE
IDQIAELIQADGLIFQDISDLVDAVREENETIQRFETSVFDGNYITADIDQEYLERIDMS
RGEASRKAPVVQAELSNLDVHNIDAAD