Protein Info for MIT1002_02491 in Alteromonas macleodii MIT1002

Annotation: Methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 675 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR00398: methionine--tRNA ligase" amino acids 7 to 533 (527 residues), 605.6 bits, see alignment E=8.5e-186 PF09334: tRNA-synt_1g" amino acids 7 to 394 (388 residues), 517.3 bits, see alignment E=5.9e-159 PF00133: tRNA-synt_1" amino acids 9 to 84 (76 residues), 33.8 bits, see alignment E=4.3e-12 PF19303: Anticodon_3" amino acids 412 to 538 (127 residues), 36.3 bits, see alignment E=1.3e-12 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 541 to 675 (135 residues), 148.6 bits, see alignment E=9.7e-48 PF01588: tRNA_bind" amino acids 580 to 672 (93 residues), 92.8 bits, see alignment E=3e-30

Best Hits

Swiss-Prot: 82% identical to SYM_PSEA6: Methionine--tRNA ligase (metG) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 99% identity to amc:MADE_01984)

MetaCyc: 63% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (675 amino acids)

>MIT1002_02491 Methionine--tRNA ligase (Alteromonas macleodii MIT1002)
MSEKRRILVTSALPYANGSIHLGHLLEHIQTDIWTRFQRLRGNECYSVCADDAHGTPVML
KAQELGITPEEMVARTRAEHHQDLVDFHVDYDNYYVTHSPENKALCEEIYTRLDNAGYIS
KRTINQLFDPEKEMFLPDRFVKGTCPSCGAEDQNGDSCDVCGATYSPTEVKNPRSVVSGA
TPVLRESEHFFFDLPKFEGMLKEWIRSGALQEEMANKLQEWFTEGLQQWDISRDAPYFGF
EIPGAPNKFFYVWVDAPVGYMASFKNFCDQNNLEFDDFWKADSEAELYHFIGKDITYFHC
LFWPAMLEGAGYRKPTGVNIHGFVTVNGAKMSKSRGTFIKGRTYLDHLNPEYLRYYFASK
LGDGVTDIDLNFTDFAQKVNSDLVGKVVNIASRCASFITKRFDGKLSENVIEPELIAEFQ
NASESIAALFEKRKYHQAVREIMALADKANQFIDNNAPWVTIKDETKQQFTHDVCSLGIN
MFRLLVIYLKPVLPVLAEKAEEFLNDKFDWNSLQTLLKGHAINKFKPMMQRVEMEKIDAM
VEDSKESLAPAAPAIDPDSPLAKDPISDTISFDEFAKVDLRIARIAKAEHVEKADKLLRL
ELDLGGETKQVFAGIKSAYSPEALIGKHTVMVANLAPRKMRFGMSEGMVLAAGPGGDELY
ILEPHEGAKPGMRVK