Protein Info for MIT1002_02465 in Alteromonas macleodii MIT1002

Annotation: Cyclic di-GMP phosphodiesterase response regulator RpfG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 509 PF11849: DUF3369" amino acids 141 to 308 (168 residues), 136.4 bits, see alignment E=1.8e-43 PF13487: HD_5" amino acids 319 to 484 (166 residues), 117.2 bits, see alignment E=1e-37 PF01966: HD" amino acids 332 to 454 (123 residues), 58.6 bits, see alignment E=1.1e-19

Best Hits

KEGG orthology group: None (inferred from 92% identity to amc:MADE_01215)

Predicted SEED Role

"Response regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (509 amino acids)

>MIT1002_02465 Cyclic di-GMP phosphodiesterase response regulator RpfG (Alteromonas macleodii MIT1002)
MSLFKKSKTEQSTSRQAPWKIAIIDDEEGVHEVTKLTLRKFNFEKRGVEFLSAHSGAEGL
ALFKANPDIALAFVDVVMETDDAGLNLVDAIRNELNNHTARLILRTGQPGQAPEEEVIRR
YDINDYKAKTELSSLKLKSCVYTAIRSYRDIQTIEQGKRGMEDVLKSSSSVLASQSLAQF
GTAVLKQTLTLLNLNNSVLYLSTQHTDLYGDTTMTVLGASGDLVGLCEPGISSRIPETIE
EEIKQVLENKSHYQSKDLFIGYYPTGEDSHNILYVKLDHPLDALQRKTLEMFASNVAVIF
QNLTQKEDIQKTQKELIMVLSDAIEMRSKETGGHVRRVILMTEFLSEKLHMSREFIETIR
YSAALHDVGKISIPETILHKPGKLDPEEWEIMKTHAQKGYELLAGSDRIVAKMGAIIAKT
HHERWDGQGYPEGLAGDDIPVEGRIMAIVDVVDALLSKRCYKPAWSPEEVKAYLHENSGK
QFDPLITHIMIEHFDRILEIRATEPDEDE