Protein Info for MIT1002_02460 in Alteromonas macleodii MIT1002

Annotation: Alcohol dehydrogenase YqhD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 transmembrane" amino acids 275 to 292 (18 residues), see Phobius details PF00465: Fe-ADH" amino acids 10 to 174 (165 residues), 145.2 bits, see alignment E=2.2e-46 PF13685: Fe-ADH_2" amino acids 19 to 109 (91 residues), 35 bits, see alignment E=2.2e-12 PF25137: ADH_Fe_C" amino acids 188 to 353 (166 residues), 101.9 bits, see alignment E=5.6e-33

Best Hits

Swiss-Prot: 59% identical to YQHD_ECOLI: Alcohol dehydrogenase YqhD (yqhD) from Escherichia coli (strain K12)

KEGG orthology group: K08325, NADP-dependent alcohol dehydrogenase [EC: 1.1.-.-] (inferred from 95% identity to amc:MADE_01218)

MetaCyc: 59% identical to NADPH-dependent aldehyde reductase YqhD (Escherichia coli K-12 substr. MG1655)
Aldehyde reductase. [EC: 1.1.1.21]; Alcohol dehydrogenase (NADP(+)). [EC: 1.1.1.21, 1.1.1.2]; 1.1.1.2 [EC: 1.1.1.21, 1.1.1.2]; 1.1.1.2 [EC: 1.1.1.21, 1.1.1.2]; 1.1.1.- [EC: 1.1.1.21, 1.1.1.2]

Predicted SEED Role

"Hypothetical oxidoreductase YqhD (EC 1.1.-.-)" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.-.- or 1.1.1.2 or 1.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (384 amino acids)

>MIT1002_02460 Alcohol dehydrogenase YqhD (Alteromonas macleodii MIT1002)
MLNFTFKNQTEILFGKGQLSEVSSRLPKQAKVLFLYGGGSIKKNGIYDKVTTALAGAEVI
EFQGVEPNPEFETLMKAVNVARENDVNFILAVGGGSVIDGAKFVAAAVNFAGDPWDILVK
GAAFKNPLPIGAVLTLPATGSESNGNSVVNRKETSQKRAFSSETVQPVFAVLDPEFTYSL
PPRQVSNGVVDAFVHVIEQYLTYPVNAPLQDRFAEGILQTLVQEGPKALATPEDYDVRAN
VMWSATMALNGLIGKGVPQDWGTHMIGHELTALHGLDHAVTLAIVLPSLMFVQRDKKQEK
ILQLGERVFGIQEDDKEKAIDLTIKAVQDFFETMQVKTRLSDHNIEKSAIDGLIENLERN
GLTALGEHGDIDLAKAREILTLAA