Protein Info for MIT1002_02424 in Alteromonas macleodii MIT1002

Annotation: 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01832: 2-deoxy-D-gluconate 3-dehydrogenase
  • amino acids 4 to 252 (249 residues), 340.9 bits, E=2.1e-106
• PF00106: adh_short
  • amino acids 9 to 202 (194 residues), 190.4 bits, E=3.8e-60
• PF08659: KR
  • amino acids 10 to 170 (161 residues), 43.7 bits, E=4.5e-15
• PF13561: adh_short_C2
  • amino acids 15 to 249 (235 residues), 193 bits, E=9.8e-61

Best Hits

Swiss-Prot: 53% identical to KDUD_DICD3: 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (kduD) from Dickeya dadantii (strain 3937)

KEGG orthology group: K00065, 2-deoxy-D-gluconate 3-dehydrogenase [EC: 1.1.1.125] (inferred from 61% identity to rmr:Rmar_2389)

MetaCyc: 53% identical to KduD (Dickeya dadantii 3937)
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase. [EC: 1.1.1.127]

Predicted SEED Role

"2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.125)

MetaCyc Pathways

• 4-deoxy-L-threo-hex-4-enopyranuronate degradation (4/5 steps found)
• 2-deoxy-D-glucose 6-phosphate degradation (2/4 steps found)
• 3,6-anhydro-α-L-galactopyranose degradation (4/7 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (12/31 steps found)

KEGG Metabolic Maps

• Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.125

Use Curated BLAST to search for 1.1.1.125 or 1.1.1.127

Protein Sequence (252 amino acids)

>MIT1002_02424 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (Alteromonas macleodii MIT1002)
MVNFSLEGKTALVTGASRGIGQALALALATSGAFVICASSRPGGCDETLRKIQSEGGQAM
ALDADLADADAVVKLAEDALSIKGHIDILLNNGGTIFRAPATDFPFDEWKNVLAVNIDSA
FLLSQTIGKTMVERGQGKIINIASMLSYTGGITVPAYTASKHAIAGLTKALANEWGQYNV
QVNAIAPGYIKTDNTQALQDDETRSAEICARIPANRWGESDDLAGAAVFLASAASNYVNG
HILAVDGGFLAR