Protein Info for MIT1002_02400 in Alteromonas macleodii MIT1002

Annotation: 4-alpha-glucanotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 733 PF21226: MalQ_N" amino acids 67 to 164 (98 residues), 66.2 bits, see alignment E=2.7e-22 TIGR00217: 4-alpha-glucanotransferase" amino acids 135 to 721 (587 residues), 607.5 bits, see alignment E=1.6e-186 PF02446: Glyco_hydro_77" amino acids 184 to 704 (521 residues), 397.2 bits, see alignment E=1.1e-122

Best Hits

KEGG orthology group: K00705, 4-alpha-glucanotransferase [EC: 2.4.1.25] (inferred from 93% identity to amc:MADE_01251)

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (733 amino acids)

>MIT1002_02400 4-alpha-glucanotransferase (Alteromonas macleodii MIT1002)
MTQQLLQQLVEMRGIETQYVDAWGKPATIAESSKAKLLNTLGYDTSSDEKIQSQITQDIK
SVWLSPLNPVQVVRNTQEINLAVRLPIELVNDDHTLTVTCENGDVLTHQFTPVDQEMTTM
AHIDDVEFHEYVVTLPLDLPLGYHDVALSADDDEFARSRLIVAPEACYTPNEIKEGKKIW
GLSVQLYCVRSEKNWGIGDFSDLALLIEKAAGVGADFIGLNPIHALYPANPNACSPYGPS
SRRWLNYLYIDVTAIDGYDDASVQAVVSSDEFKATLEHARNVEHVDYEAVAHVKLAALKA
VFDVYDAKYLRKSTKQNKAFKAFVEAGGESLDMLAVYDALQSHLKAEGKDSWGWPVFPQD
YKDYYNPAVAKFKSANEQDVKFYLFLQWIAAQQLELASNKATDAGMTIGLYRDLAVGVSE
GSAEIWGNKDLYCTGASVGAPPDILGPLGQNWGLPPMDPRKLYEQGYQPIIDLFASNMAS
SGSLRIDHVMALLRLWWVVKGDNAKDGGYVYYPVDDLLGILALESHRNQSLVIGEDLGTV
PEEIRSKLADNGVYSYRVFFFEQAEDGGFFSPSHYPVQSMSTLTTHDMPTLIGYWHCLDL
ELGKEIGLYPTEEILQTLYADRHENKQAILDTLHGHGSIGDNVGRDVNHTGMNRDLNNGM
QVHMAGGSSALLSLQLEDWLEMDKPVNIPGTFDEYPNWRRKLTENIESMFDRHDINELAT
KLTHARKRASQGE