Protein Info for MIT1002_01964 in Alteromonas macleodii MIT1002

Annotation: Glutamine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 555 TIGR00440: glutamine--tRNA ligase" amino acids 28 to 552 (525 residues), 897.6 bits, see alignment E=1.6e-274 PF00749: tRNA-synt_1c" amino acids 28 to 337 (310 residues), 394 bits, see alignment E=6.4e-122 PF03950: tRNA-synt_1c_C" amino acids 340 to 440 (101 residues), 111.8 bits, see alignment E=2.2e-36 PF20974: tRNA-synt_1c_C2" amino acids 457 to 530 (74 residues), 71.5 bits, see alignment E=1.2e-23

Best Hits

Swiss-Prot: 96% identical to SYQ_ALTMD: Glutamine--tRNA ligase (glnS) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 96% identity to amc:MADE_01892)

MetaCyc: 72% identical to glutamine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glutamine--tRNA ligase. [EC: 6.1.1.18]

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (555 amino acids)

>MIT1002_01964 Glutamine--tRNA ligase (Alteromonas macleodii MIT1002)
MAETEHRPTNFIRQIIDKDLSSGLHDAIKTRFPPEPNGFLHIGHAKSICLNFGIAQDYTG
TCNLRFDDTNPAKEDITFVNSIKEDVQWLGFTWDGEARYSSNYFDQLHAYAVELIEKGLA
YVDFSAQDVMREMRGTLKEPGQNSPYRDTDVETNLSEFAKMTAGEYKEGECSLRAKIDMA
SPFMCMRDPVIYRVKHAHHHQTGDKWCVYPMYDFTHCISDAIEGITHSLCTLEFQDNRRL
YDWVIENISIDCTPRQYEFSRLNLEYTVLSKRRLIQLVDENHVHGWDDPRMPTIAGLRRR
GYTPGSIVEFCKRIGVTKMDNMVEMSMLEACIRDDLNANAPRAMAVLDPIKLVIENYEEG
QTETLVAPNHPNDESMGTRNIGFSREVYIEAEDFRESANKKFKRLVLDKEVRLRNAYVVK
ANRVEKDENGNVTTVFCTYDADTLGKDPADGRKVKGVIHWVDAATAQAAEFRLYDRLFNV
PNPAAEENFTDAINPDSLEVKHGWVEAGLADNVPEVGAWQFERTGYFCVDKDSTDDKPVF
NRTVGLRDTWAKMGE