Protein Info for MIT1002_01888 in Alteromonas macleodii MIT1002

Annotation: Polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 703 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 9 to 690 (682 residues), 1082.8 bits, see alignment E=0 PF01138: RNase_PH" amino acids 14 to 143 (130 residues), 106.7 bits, see alignment E=3.1e-34 amino acids 323 to 455 (133 residues), 99.9 bits, see alignment E=3.8e-32 PF03725: RNase_PH_C" amino acids 146 to 209 (64 residues), 49.2 bits, see alignment E=1e-16 amino acids 460 to 528 (69 residues), 25.2 bits, see alignment E=3.1e-09 PF03726: PNPase" amino acids 241 to 319 (79 residues), 74.7 bits, see alignment E=1.7e-24 PF00013: KH_1" amino acids 555 to 613 (59 residues), 40.3 bits, see alignment 5.5e-14 PF00575: S1" amino acids 618 to 689 (72 residues), 65.8 bits, see alignment E=9.3e-22

Best Hits

Swiss-Prot: 86% identical to PNP_PSEA6: Polyribonucleotide nucleotidyltransferase (pnp) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 94% identity to alt:ambt_09400)

MetaCyc: 71% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (703 amino acids)

>MIT1002_01888 Polyribonucleotide nucleotidyltransferase (Alteromonas macleodii MIT1002)
MTPITKSFQYGQHTVTLETGVIARQATAAVLASMDDTSVLVTVVGKKEAKADQDFFPLTV
NYQEKAYAAGKIPGGFFKREGRPSEGETLIARLIDRPIRPLFPEGFKNEVQVVVTVMSAN
PDIPTDIISMIGTSAALAISGIPFNGPIGAARVGFSNGEYILNTRAEEQAESELDLVVAG
TEGAVLMVESEASVLSEDTMLGAVMFGHEQMQTVVNAVNEFAAEVGTEKWDWAPEAENTA
LKDKVKALAEAEMTAAYQISDKLERKDAVTAATEKALEAILAEDEEQDKKEVLDLLHDLE
SDVVRSRILSGAPRIDGRDPAMIRALDVATGILPRTHGSALFTRGETQALVAATLGTERD
AQMIDELQGKRDSRFMLHYNFPPYCVGETGMIGSPKRREIGHGRLAKRGIQAVMPSEEEF
PYVVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKASVAGIAMGLVKSDDNFVVLSDI
LGDEDHLGDMDFKVAGTTEGITALQMDIKIEGITKEIMQIALKQAKEARLHILNVMDEAI
GGHREELSEFAPRIYTMKIDQDKIRDVIGKGGAMIRQITEESDTNIEIEDDGTIKIFATE
RAKADIAISKIEQVTAEIEVGKTYNGKITRIVDFGAFVEVLPGKEGLVHISQIAHERVNK
VTDYLEEGQMVDVKVMEIDRQNRVRLSIKELLEKPAPKSDDAE