Protein Info for MIT1002_01857 in Alteromonas macleodii MIT1002

Annotation: Leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 867 TIGR00396: leucine--tRNA ligase" amino acids 6 to 866 (861 residues), 1237.1 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 15 to 226 (212 residues), 111.6 bits, see alignment E=7.9e-36 amino acids 423 to 578 (156 residues), 35.8 bits, see alignment E=8.2e-13 amino acids 625 to 661 (37 residues), 28.6 bits, see alignment (E = 1.3e-10) PF09334: tRNA-synt_1g" amino acids 40 to 172 (133 residues), 71.3 bits, see alignment E=1.4e-23 PF13603: tRNA-synt_1_2" amino acids 223 to 410 (188 residues), 259.2 bits, see alignment E=3.4e-81 PF08264: Anticodon_1" amino acids 707 to 830 (124 residues), 79.9 bits, see alignment E=3.7e-26

Best Hits

Swiss-Prot: 69% identical to SYL_COLP3: Leucine--tRNA ligase (leuS) from Colwellia psychrerythraea (strain 34H / ATCC BAA-681)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 98% identity to amc:MADE_01672)

MetaCyc: 67% identical to leucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Leucine--tRNA ligase. [EC: 6.1.1.4]; 6.1.1.4 [EC: 6.1.1.4]; 3.1.1.- [EC: 6.1.1.4]

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (867 amino acids)

>MIT1002_01857 Leucine--tRNA ligase (Alteromonas macleodii MIT1002)
MADNQYNPKDIEQRVQQYWEENQSFKANDDVEKEKFYCLSMFPYPSGRLHMGHVRNYTIG
DVISRFQRMQGKNVLQPMGWDAFGLPAENAAINNNTAPAKWTYSNIDYMKNQLRSLGFGY
DWDREVTTCKSDYYRWEQWFFTRLYEKGLVYKKNSTVNWDPVDQTVLANEQVIDGRGWRS
GALVEQKEIPQWFIKITDYAEELLQDLEQLEEWPDQVRAMQANWIGRSEGVEITFDLASA
VNDIADLTVYTTRPDTFYGVTYVAVAAQHPLAEFAAKSNPDLAAFIDECKNTKVAEAELA
TMEKKGFATGFEVVHPLTGEKLPIWVANFVLMDYGSGAVMAVPGHDQRDWEFATKYNLPI
QQVIAPNAGDEDKCDLTASAFTEKGSLINSAKFDGLEFDSAFNGIADELESKGCGKRKVN
YRLRDWGVSRQRYWGAPIPMLNLENGESVPVPADQLPVVLPEDVEMNGVTSPIKADPEWA
KTTYNGEAALRETDTFDTFMESSWYYARYACATNNDAMLDPTSANYWLPVDQYIGGIEHA
ILHLLYSRFFHKLLRDEGLVKSDEPFKRLLCQGMVLADSYYREDASGKKTWFSPTEVSTE
KDDKGRIVKAWLTADGEEVIHGGMTKMSKSKNNGIDPQEVIDQYGADTVRLFTMFAAPPE
QTLEWVDSGVEGANRFLRRIWKLVTEHVEKGAPEAIDVKALSKDQQALRREVHKTIEKVS
DDLGRRQTFNTAVAAVMELLNHLQKAPQENAQDIAIMREACESILLLLNPITPHIAHELW
KVLGHSEDIDLAPWPQADKAALVEDEKLIIVQVNGKVRAKMTIAADASKESIEASAKEQP
NVQQFIEGKTIRKVIVVPGKLVNIVAN