Protein Info for MIT1002_01826 in Alteromonas macleodii MIT1002

Annotation: Adenylosuccinate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00928: adenylosuccinate lyase
  • amino acids 12 to 455 (444 residues), 470.3 bits, E=2.9e-145
• PF00206: Lyase_1
  • amino acids 14 to 312 (299 residues), 234 bits, E=2.9e-73
• PF08328: ASL_C
  • amino acids 331 to 445 (115 residues), 174.8 bits, E=5.8e-56

Best Hits

Swiss-Prot: 74% identical to PUR8_ECOLI: Adenylosuccinate lyase (purB) from Escherichia coli (strain K12)

KEGG orthology group: K01756, adenylosuccinate lyase [EC: 4.3.2.2] (inferred from 97% identity to amc:MADE_02370)

MetaCyc: 74% identical to adenylosuccinate lyase (Escherichia coli K-12 substr. MG1655)
Adenylosuccinate lyase. [EC: 4.3.2.2]; 4.3.2.2 [EC: 4.3.2.2]

Predicted SEED Role

"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of purine nucleotide salvage (12/14 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)
• dZTP biosynthesis (4/5 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.2.2

Protein Sequence (455 amino acids)

>MIT1002_01826 Adenylosuccinate lyase (Alteromonas macleodii MIT1002)
MELSQLTAISPVDGRYAGKSVELRSIFSEYGLLKYRVEVEVRWLQMLSTNPQIEEVPAFT
DTSNALLDKIVAEFSVEDAMRIKEIERTTNHDVKAVEYFLKEKVADNSELSAVNEFIHFA
CTSEDINNLSHGLMLREARETVILPYCDKLIEALVELAKRYQNVPMMARTHGQPASPTTM
GKEMANVAIRLKRQRSQIAGVELLGKINGAVGNYNAHLSAYKDVDWHEVSEKFVTSLGLT
WNPFTTQIEPHDYIAEMFDAIARFNTILIDFDRDVWGYIALGHFKQKTVAGEIGSSTMPH
KVNPIDFENSEGNLGLANAIFDHLAAKLPISRWQRDLTDSTVLRNLGVGVGYAVIAYQAT
LKGISKLEVNEQSLLNELNNNWELLAEPIQTVMRRYGIEKPYEKLKELTRGKKVNAEVIA
EFIDNLDMPEAAKAELKALTPATYIGDAIRLVDQL