Protein Info for MIT1002_01666 in Alteromonas macleodii MIT1002

Annotation: Acetyl-/propionyl-coenzyme A carboxylase alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 152.1 bits, see alignment E=2.7e-48 PF02786: CPSase_L_D2" amino acids 116 to 323 (208 residues), 244.5 bits, see alignment E=2.9e-76 PF02222: ATP-grasp" amino acids 123 to 292 (170 residues), 42.5 bits, see alignment E=2e-14 PF07478: Dala_Dala_lig_C" amino acids 140 to 291 (152 residues), 42.2 bits, see alignment E=2.4e-14 PF02785: Biotin_carb_C" amino acids 336 to 443 (108 residues), 130.7 bits, see alignment E=8.3e-42 PF21139: BT_MCC_alpha" amino acids 469 to 576 (108 residues), 31.3 bits, see alignment E=8.2e-11 PF00364: Biotin_lipoyl" amino acids 599 to 662 (64 residues), 48.6 bits, see alignment E=2e-16

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 81% identity to amc:MADE_02415)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (672 amino acids)

>MIT1002_01666 Acetyl-/propionyl-coenzyme A carboxylase alpha chain (Alteromonas macleodii MIT1002)
MINKLLIANRGEIACRVIKTANAQGIKTVAVYSDADKNALHVQMADEAVYLGPSPSKKSY
LRGELIIEKAKELGVDAIHPGYGFLSENAEFANLCAKNNIIFVGPPASAIEAMGSKSAAK
HIMEKAGVPLVPGYHGDDQSEAVLKGAADEMGYPVLLKAAAGGGGKGMRQVWSEKEFSQA
LNAAKRESMASFGDDHMLVEKYLTRPRHVEIQVFCDTHGNGVYLFERDCSVQRRHQKIIE
EAPAPNMSQSVREKMGEAAILAAKAINYVGAGTVEFLLDEDDSFYFMEMNTRLQVEHPVT
EMITREDLVHWQLTIAEGKPLPKQQNELTLTGHAFEARIYAEDPNNEFLPSTGTLRLLRT
PKENDVVRVDTGVVEGDEVSVFYDPMIAKLVVWGENREIALKRLISALGDYYIDGVSTNI
DFLKRVATHPAFVAAELTTTFVEKHHDSLFSATSEDAASDDTQVNIPVMALLSLLNRKVS
RSDKTPSVWSTVGAWRANANHTEILTLLCNQEEVHVGVKHQRHGTEDAWELSVGDNSYNV
KGKLVDSALHATINGYKSTFTYSDNDGVFTLFNKDTHAKFSLISASLGDDNDDNGDANFS
APMNGTIVAHLVEKGVLVKKGEPILVMEAMKMEHSIIAPHDGAVEEFFFNPGELVDGGAT
LLAFSTKEAAEV