Protein Info for MIT1002_01509 in Alteromonas macleodii MIT1002

Annotation: Ribose-phosphate pyrophosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 315 PF13793: Pribosyltran_N" amino acids 4 to 121 (118 residues), 179.9 bits, see alignment E=2.1e-57 TIGR01251: ribose-phosphate diphosphokinase" amino acids 4 to 314 (311 residues), 410 bits, see alignment E=2.6e-127 PF00156: Pribosyltran" amino acids 160 to 251 (92 residues), 64.9 bits, see alignment E=8.6e-22 PF14572: Pribosyl_synth" amino acids 203 to 313 (111 residues), 110.7 bits, see alignment E=1.3e-35

Best Hits

Swiss-Prot: 81% identical to KPRS_PHOLL: Ribose-phosphate pyrophosphokinase (prs) from Photorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)

KEGG orthology group: K00948, ribose-phosphate pyrophosphokinase [EC: 2.7.6.1] (inferred from 100% identity to amc:MADE_02577)

MetaCyc: 81% identical to ribose-phosphate diphosphokinase (Escherichia coli K-12 substr. MG1655)
Ribose-phosphate diphosphokinase. [EC: 2.7.6.1]

Predicted SEED Role

"Ribose-phosphate pyrophosphokinase (EC 2.7.6.1)" in subsystem De Novo Purine Biosynthesis or Pentose phosphate pathway (EC 2.7.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (315 amino acids)

>MIT1002_01509 Ribose-phosphate pyrophosphokinase (Alteromonas macleodii MIT1002)
MPDMKLFAGNAVPELAQKVADRLYTKLGNAKVGRFSDGEISVEIHENVRGSDVFIIQSTC
APTNDNLMELIVMIDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSNV
GVDRVLTIDLHAEQIQGFFDVPVDNAFGTPILLADMVRRDFADPVVVSPDIGGVVRARAT
AKLLNDTDLAIIDKRRPKANVAQVMNIIGDVKDRDCIIVDDMIDTGGTLAKAAEALKAHG
ARRVYAYATHAIFSGNAANNLKESVIDEIIVTDSIPLSAEMKQIGKVKQLTLSEMLAETI
RRISNEESISAMFEY