Protein Info for MIT1002_01427 in Alteromonas macleodii MIT1002

Annotation: Tryptophan biosynthesis protein TrpCF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00218: IGPS
  • amino acids 5 to 255 (251 residues), 292.7 bits, E=2.1e-91
• PF00697: PRAI
  • amino acids 260 to 463 (204 residues), 168.1 bits, E=1.9e-53

Best Hits

Swiss-Prot: 55% identical to TRPC_VIBCH: Tryptophan biosynthesis protein TrpCF (trpCF) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K13498, indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase [EC: 4.1.1.48 5.3.1.24] (inferred from 86% identity to amc:MADE_02291)

MetaCyc: 53% identical to fused indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylanthranilate isomerase. [EC: 5.3.1.24]; Indole-3-glycerol-phosphate synthase. [EC: 5.3.1.24, 4.1.1.48]

Predicted SEED Role

"Indole-3-glycerol phosphate synthase (EC 4.1.1.48) / Phosphoribosylanthranilate isomerase (EC 5.3.1.24)" in subsystem Tryptophan synthesis or Auxin biosynthesis (EC 4.1.1.48, EC 5.3.1.24)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• L-tryptophan biosynthesis (6/6 steps found)
• superpathway of chorismate metabolism (42/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.48 or 5.3.1.24

Protein Sequence (469 amino acids)

>MIT1002_01427 Tryptophan biosynthesis protein TrpCF (Alteromonas macleodii MIT1002)
MANVLEKIVADKREEVAARKEALPLESFKANLVPSEKSLFAALSEPNAGFIFECKKASPS
KGLIREHFDLDEILAAYTPYAAGISVLTDEKYFQGKFEYLAYVTERIAQPVLNKDFFVDT
YQVYLARHYNADAVLLMLSVLNDDEYRELASVANGLSLDILTEVSNEEEMERAIALEANI
IGINNRNLRDLSTDLATTERLVPMLEKATHDYVVISESGIYTHQDVLRLAPVSQGFLVGS
ALMAEADLPRAVKTLVNGAVKVCGLTNPEQAQMAFDKGASFGGLIFAEKSPRYVSEAQAL
TITQSIDGAFVGVFVNHDIDEVASLAALLNLFAVQLHGSEDDTYISALATKLPEGCEIWK
VEGVKATSSGALPDAVDRHLNNSLVSRVLLDCQVGDAKGGTGEAFNWQLLNDIEAKHKLV
LAGGINAQNVAEAIATGCGAIDVNSGVETAPGVKSEEKLDALFAICRRY