Protein Info for MIT1002_01395 in Alteromonas macleodii MIT1002

Annotation: Cyclic pyranopterin monophosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 322 TIGR02666: molybdenum cofactor biosynthesis protein A" amino acids 2 to 322 (321 residues), 325.3 bits, see alignment E=2e-101 PF04055: Radical_SAM" amino acids 16 to 175 (160 residues), 100.8 bits, see alignment E=1.4e-32 PF13353: Fer4_12" amino acids 19 to 120 (102 residues), 32 bits, see alignment E=2.3e-11 PF06463: Mob_synth_C" amino acids 181 to 305 (125 residues), 111 bits, see alignment E=6.1e-36

Best Hits

Swiss-Prot: 55% identical to MOAA_YERPE: GTP 3',8-cyclase (moaA) from Yersinia pestis

KEGG orthology group: K03639, molybdenum cofactor biosynthesis protein (inferred from 95% identity to amc:MADE_02199)

MetaCyc: 54% identical to GTP 3',8'-cyclase (Escherichia coli K-12 substr. MG1655)
RXN-8340 [EC: 4.1.99.22]

Predicted SEED Role

"Molybdenum cofactor biosynthesis protein MoaA" in subsystem Molybdenum cofactor biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (322 amino acids)

>MIT1002_01395 Cyclic pyranopterin monophosphate synthase (Alteromonas macleodii MIT1002)
MLEDSFGRQFHYLRLSVTEACNFRCQYCLPDGYEGPTSDQFMTLNEIDTLLKAFAKLGTS
KVRLTGGEPTLRRDFLDILQLTSNTPGIERVAMTTHGARMEKFAHQWKVAGLHQVNVSID
SLDPRQFAAITGQDKLKAVLRGLDAAIDAGLDVKVNSVLLNDFSDSRLHRFLAWLKDMPV
TLRFIELMETGDLNQFFNKQHQSGTPLKSSLLDMGWQPLLRRKDAGPAQEFYHPDYAGKI
GLIMPYSKDFCKTCNRLRVSAPGKLHLCLFSENGIDMRHLLATGNVDEVVAFLQDVLGQK
HETHYLHEGKTGATKHLAMLGG