Protein Info for MIT1002_01310 in Alteromonas macleodii MIT1002

Annotation: tRNA (guanine-N(1)-)-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 253 TIGR00088: tRNA (guanine(37)-N(1))-methyltransferase" amino acids 6 to 238 (233 residues), 340.2 bits, see alignment E=2.4e-106 PF01746: tRNA_m1G_MT" amino acids 27 to 225 (199 residues), 223.4 bits, see alignment E=1.1e-70

Best Hits

Swiss-Prot: 75% identical to TRMD_PSEA6: tRNA (guanine-N(1)-)-methyltransferase (trmD) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K00554, tRNA (guanine-N1-)-methyltransferase [EC: 2.1.1.31] (inferred from 94% identity to alt:ambt_12235)

MetaCyc: 71% identical to tRNA m1G37 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-12458 [EC: 2.1.1.228]

Predicted SEED Role

"tRNA (Guanine37-N1) -methyltransferase (EC 2.1.1.31)" in subsystem Ribosome biogenesis bacterial or Wyeosine-MimG Biosynthesis (EC 2.1.1.31)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.228 or 2.1.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (253 amino acids)

>MIT1002_01310 tRNA (guanine-N(1)-)-methyltransferase (Alteromonas macleodii MIT1002)
MTPEKWFGVVSLFPDMFAPFTQQGVIGRAVKSGTLSVDTFNPRDFTHDRHRTVDDRPYGG
GPGMLMMVKPLTDAIQAAKKVGGEKSKVIYLSPQGKPLDQAGVQRLANIDRTILICGRYE
GIDERVIESHVDEEVSIGDYVLSGGELPAMVLMDAVARLVPGVLGHKASAVEDSFTDGLL
DCPHYTRPEILDGQKVPDVLLSGNHEKIRQWRLMQSLGRTWQRRPELLNHLALTEEQQRL
LELFQAQLQQDDS