Protein Info for MIT1002_01271 in Alteromonas macleodii MIT1002

Annotation: Sulfate adenylyltransferase subunit 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 23 to 229 (207 residues), 161 bits, E=3.8e-51
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 25 to 430 (406 residues), 657.2 bits, E=1e-201
• TIGR00231: small GTP-binding protein domain
  • amino acids 31 to 210 (180 residues), 49 bits, E=5.8e-17
• PF01926: MMR_HSR1
  • amino acids 85 to 166 (82 residues), 23.7 bits, E=6.5e-09
• PF22594: GTP-eEF1A_C
  • amino acids 332 to 430 (99 residues), 98.2 bits, E=4.8e-32

Best Hits

Swiss-Prot: 100% identical to CYSN_ALTMD: Sulfate adenylyltransferase subunit 1 (cysN) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K00956, sulfate adenylyltransferase subunit 1 [EC: 2.7.7.4] (inferred from 100% identity to amc:MADE_02716)

MetaCyc: 60% identical to sulfate adenylyltransferase subunit 1 (Escherichia coli K-12 substr. MG1655)
Sulfate adenylyltransferase. [EC: 2.7.7.4]

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4)" in subsystem Cysteine Biosynthesis (EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (2/12 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.7.4

Protein Sequence (471 amino acids)

>MIT1002_01271 Sulfate adenylyltransferase subunit 1 (Alteromonas macleodii MIT1002)
MNNENELLRQDILSYLEQHEKKDMLRFLTCGSVDDGKSTLIGRLLHDSKMIYEDQLAAIT
KDSKKVGTTGEKVDLALLVDGLQSEREQGITIDVAYRYFSTDKRKFIIADTPGHEQYTRN
MVTGASTCDLAIILVDARGGVKVQTKRHSFLVSLLGIKHVIVAINKMDLMDYSEEVYKQI
QEDYLKFAEQLDIPDIQFVPISALEGDNVVGKSEKTPWFDGTPLMEMLENIEIGEDDNLE
DFRFPVQYVNRPNLDFRGFAGTVVSGQVAPGDEVTALPSGKKSKVKQVVTFEGDQERAYV
PQAVTLTLEDEIDISRGDMIVKSDNLPLLNTQFKTHLVWMSEEPLMPNKQYLFKFATKST
PGVVAHIDNQIDVNTLEETDAMHLNLNEIGVVDVKFTQPVACDPYKRNRPTGSFIVIDRL
TNGTVGAGMIIDEIAGDAQHTSPNFSEFELEFNALVRKHFPHWNSVDISKL