Protein Info for MIT1002_01266 in Alteromonas macleodii MIT1002

Annotation: Asparagine synthetase B [glutamine-hydrolyzing]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 71% identical to ASNB_ECOLI: Asparagine synthetase B [glutamine-hydrolyzing] (asnB) from Escherichia coli (strain K12)

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 99% identity to amc:MADE_02722)

MetaCyc: 71% identical to asparagine synthetase B (Escherichia coli K-12 substr. MG1655)
Asparagine synthase (glutamine-hydrolyzing). [EC: 6.3.5.4]; Aspartate--ammonia ligase. [EC: 6.3.5.4, 6.3.1.1]; Glutamate synthase (NADPH). [EC: 6.3.5.4, 6.3.1.1, 1.4.1.13, 1.4.1.14, 1.4.7.1, 2.6.1.85, 3.5.1.2, 3.5.1.38, 4.1.3.27, 4.3.2.10, 4.3.3.6, 6.3.4.2, 6.3.5.13, 6.3.5.2, 6.3.5.5, 6.3.5.6, 6.3.5.7]

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
superpathway of aromatic amino acid biosynthesis (18/18 steps found)
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
superpathway of L-tryptophan biosynthesis (13/13 steps found)
L-histidine biosynthesis (10/10 steps found)
superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
superpathway of purine nucleotide salvage (12/14 steps found)
L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
superpathway of pyrimidine ribonucleosides salvage (9/10 steps found)
superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
L-tryptophan biosynthesis (6/6 steps found)
UMP biosynthesis I (6/6 steps found)
UMP biosynthesis II (6/6 steps found)
superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
nitrogen remobilization from senescing leaves (7/8 steps found)
superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
superpathway of pyrimidine nucleobases salvage (4/4 steps found)
L-glutamate and L-glutamine biosynthesis (6/7 steps found)
L-citrulline degradation (3/3 steps found)
UTP and CTP de novo biosynthesis (3/3 steps found)
ammonia assimilation cycle III (3/3 steps found)
superpathway of ammonia assimilation (plants) (3/3 steps found)
UMP biosynthesis III (5/6 steps found)
4-aminobenzoate biosynthesis I (2/2 steps found)
L-glutamate biosynthesis I (2/2 steps found)
ammonia assimilation cycle I (2/2 steps found)
ammonia assimilation cycle II (2/2 steps found)
superpathway of L-asparagine biosynthesis (2/2 steps found)
L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
L-asparagine biosynthesis I (1/1 steps found)
L-asparagine biosynthesis II (1/1 steps found)
L-asparagine degradation I (1/1 steps found)
L-glutamate biosynthesis IV (1/1 steps found)
L-glutamate biosynthesis V (1/1 steps found)
L-glutamine degradation I (1/1 steps found)
L-glutamine degradation II (1/1 steps found)
L-citrulline biosynthesis (6/8 steps found)
GABA shunt II (3/4 steps found)
L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
UTP and CTP dephosphorylation I (5/7 steps found)
L-asparagine degradation III (mammalian) (2/3 steps found)
superpathway of chorismate metabolism (42/59 steps found)
superpathway of L-citrulline metabolism (8/12 steps found)
UTP and CTP dephosphorylation II (1/3 steps found)
peptidoglycan biosynthesis II (staphylococci) (11/17 steps found)
peptidoglycan biosynthesis IV (Enterococcus faecium) (11/17 steps found)
4-hydroxy-2(1H)-quinolone biosynthesis (2/5 steps found)
acridone alkaloid biosynthesis (1/4 steps found)
L-arginine biosynthesis IV (archaea) (4/9 steps found)
superpathway of arginine and polyamine biosynthesis (9/17 steps found)
allantoin degradation IV (anaerobic) (3/9 steps found)
superpathway of candicidin biosynthesis (3/11 steps found)
superpathway of quinolone and alkylquinolone biosynthesis (2/10 steps found)
chloramphenicol biosynthesis (1/9 steps found)
guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14, 1.4.7.1, 2.6.1.85, 3.5.1.2, 4.1.3.27, 6.3.4.2, 6.3.5.2, 6.3.5.4, 6.3.5.5, 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14 or 1.4.7.1 or 2.6.1.85 or 3.5.1.2 or 3.5.1.38 or 4.1.3.27 or 4.3.2.10 or 4.3.3.6 or 6.3.1.1 or 6.3.4.2 or 6.3.5.13 or 6.3.5.2 or 6.3.5.4 or 6.3.5.5 or 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (556 amino acids)

>MIT1002_01266 Asparagine synthetase B [glutamine-hydrolyzing] (Alteromonas macleodii MIT1002)
MCGIFGILDIKTDVSELRTQALDLSKLLRHRGPDWSGIWNNDNTILCHERLAIVDVDTGA
QPLISQNDKQILAVNGEIYNHKQLAANLDEPYPFKTRSDCEVILPLFQQKGIDFIDELEG
MFAFILYDEEQDAYLIARDHIGIIPLYTGYDEHGNFYVSSEMKALAGVCKTISEFPPGHY
LWSKEGKITKYYKRDWMEYDNVKDNTSNLEELRVAFEKAVKSHMMSDVPYAVLLSGGLDS
SLVSAVAAQYVAKRVEDEDKTDAWWPRLHSFAVGLEGAPDLKAAKKVADMIGTVHHEIHF
TIQEGLDAIRDVIYHLETYDTTTIRAATPMYLMTRKIKAMGIKMVLSGEGSDEIFGGYLY
FHKAPNAKEFHEETVRKLDRLHMFDCARANKATSAWGVEARVPFLDKNFIDVAMRLNPQD
KMCLDGKMEKWILRKAFDNGETLPAEVLWRQKEQFGDGVGYSWIDSIRDFVENEVTDQQL
ATAEFRFPVNTPDTKEGYYYRTIFESYFPQESAARCVPGGKSIACSTAEALEWDESFKNN
ADPSGRSMKGVHAGES