Protein Info for MIT1002_01222 in Alteromonas macleodii MIT1002

Annotation: Glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 532 TIGR01434: glutamate--cysteine ligase" amino acids 11 to 522 (512 residues), 619.5 bits, see alignment E=2.2e-190 PF04262: Glu_cys_ligase" amino acids 13 to 385 (373 residues), 548.7 bits, see alignment E=2.7e-169

Best Hits

Swiss-Prot: 48% identical to GSH1_SHEAM: Glutamate--cysteine ligase (gshA) from Shewanella amazonensis (strain ATCC BAA-1098 / SB2B)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 92% identity to amc:MADE_02763)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (532 amino acids)

>MIT1002_01222 Glutamate--cysteine ligase (Alteromonas macleodii MIT1002)
MTVDSSRFEARIAALKSPEFLAALKDIKRGVEREALRINPNGTLAQTPHPKPLGSALTHD
SITTDFSESLLEFITPPENSAAKTISQLKDIHKFVIDNIGEEQIWPLSMPCFIDDEAHIP
IAYFGESNVGKMKRVYRIGLKNRYGSMMQAIAGVHFNFSLPETFWKLWAELNGKEHSQEQ
TSADYFGLIRNYRRLCWLIPYLYGASPALCGSFLKGKQHALPFKKVGKGTVYMPYATSLR
MSDLGYTSAEQSSLKICYNKLDNYVTLLRDAMNTPSSRFSQFAAGEEGNYQQLSRNIIQI
ENELYSPIRPKQPTQSMEKPTDALVRRGISYIEVRALDVNPFSAIGISQTQFDFLDVFLV
ACLLMPSAELDEAQLKEAKENMNKVVLEGRNPDLLLQNGGENISLPNWCASLFKSFEQAA
ELLDLANDTSRYTQAVNLEAEKVADPALTPSGKLLATLLESDKDNGVLGLELAQAFQAEM
KSFEYTDTDAAGFAAQADVSFAAQKEIEATDVKDFDTFIRDYFAEAPAKKNA