Protein Info for MIT1002_01216 in Alteromonas macleodii MIT1002

Annotation: Lysine--tRNA ligase, heat inducible

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 TIGR00499: lysine--tRNA ligase" amino acids 9 to 510 (502 residues), 656.1 bits, see alignment E=1.6e-201 PF01336: tRNA_anti-codon" amino acids 63 to 135 (73 residues), 42.3 bits, see alignment E=6e-15 PF00152: tRNA-synt_2" amino acids 151 to 508 (358 residues), 318.6 bits, see alignment E=4.3e-99

Best Hits

Swiss-Prot: 73% identical to SYK_COLP3: Lysine--tRNA ligase (lysS) from Colwellia psychrerythraea (strain 34H / ATCC BAA-681)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 99% identity to amc:MADE_02769)

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (522 amino acids)

>MIT1002_01216 Lysine--tRNA ligase, heat inducible (Alteromonas macleodii MIT1002)
MTDQQTDDNKLIAERRAKLSAIREQCRANGFPNSFRRENYADELQAKFGEFDKETLQEQG
NKVSIAGRIMAKRGPFMLLQDMTGRIQAYADKDTQKALKAKYGALDIGDIIGVAGDLHKS
GKGDLYVNMEQYELLTKALRPLPEKFHGLSDQETKYRQRYVDLITSEQTRKTFMIRSKIV
NGIRNYLTKRDYLEVETPMLQVIPGGATAKPFVTHHNALDIDMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSTRHNPEFTMLEFYQAYADYNDLMNLTEDMLRTLAEDILGTSII
KNTNKDAEGNVTSEVEYDFGKPFDRLSMGEAILKYWPEANEEAIRDPEANLDALKAMAKQ
LHIKEPEVDGIWGAGKYLCEIFEATAEEKLIQPTFITEYPWEVSPLARRNDNNPFITDRF
EFFVGGRELANGFSELNDPEDQAERFAKQVEEKDAGDDEAMHFDDDYIRALEYGLPPTAG
EGIGIDRLAMLFTDSSTIKDVILFPHMKPQAPKDAQEAGEQE