Protein Info for MIT1002_01193 in Alteromonas macleodii MIT1002

Annotation: Arginine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 576 TIGR00456: arginine--tRNA ligase" amino acids 3 to 576 (574 residues), 579.2 bits, see alignment E=4.1e-178 PF03485: Arg_tRNA_synt_N" amino acids 9 to 86 (78 residues), 80.8 bits, see alignment E=1.4e-26 PF00750: tRNA-synt_1d" amino acids 102 to 445 (344 residues), 446.2 bits, see alignment E=1.2e-137 PF05746: DALR_1" amino acids 459 to 576 (118 residues), 107.9 bits, see alignment E=5.1e-35

Best Hits

Swiss-Prot: 97% identical to SYR_ALTMD: Arginine--tRNA ligase (argS) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 97% identity to amc:MADE_02786)

MetaCyc: 58% identical to arginine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Arginine--tRNA ligase. [EC: 6.1.1.19]

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (576 amino acids)

>MIT1002_01193 Arginine--tRNA ligase (Alteromonas macleodii MIT1002)
MNIHALLVNRFTEALQEMGVENAPVPVSRSARPEFGEYQFNGAMALAKQLKQKPRDIAEK
IVETVKLDDIASKLEVAGPGFINVHLNDEWLSNQCELSLTDPRLGIAKSKEQNIVVDYSS
PNLAKEMHVGHLRTTIIGDAVVKVLEFLGHNVIRQNHMGDWGTQFGMLLAHLSDKLQDEV
AETALSDLEDFYREAKVRFDEEEGFADRAREYVVKLQGGDAQCLALWEKFIDVSIAHSEE
VYDKLNVSLTRKDIMGESAYNDDLANVISDLKAKGLAVEDQGAQVVFIPELADKEGNPAV
YIVQKSGGGYLYATTDLAAMRYRSGKLNADRTLILTDARQALHFKQTEIVGRKAGFMKEE
QTYEHCPFGMMLGSDGKPFKTRTGGTVKLVELLDEAVERAGKLIAERDNDLSEDELKEVA
HKVGIGAVKYADLSKNRTTDYMFNWDSMLSFEGNTAPYLQYAYTRVKSLFRKAGVDMATM
PVDINIAEKQEHALAVLLMQFEEVIGTVSREATPHVLCTYLYDVASAFMSFYEACPMLKD
GIEPAVRDSRLALSALVAKTLEQGLTLLGIETLEKM