Protein Info for MIT1002_01112 in Alteromonas macleodii MIT1002

Annotation: Homoserine O-succinyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 97% identical to METAS_ALTMD: Homoserine O-succinyltransferase (metAS) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K00651, homoserine O-succinyltransferase [EC: 2.3.1.46] (inferred from 97% identity to amc:MADE_02861)

MetaCyc: 54% identical to homoserine O-succinyltransferase (Escherichia coli K-12 substr. MG1655)
Homoserine O-succinyltransferase. [EC: 2.3.1.46]

Predicted SEED Role

"Homoserine O-succinyltransferase (EC 2.3.1.46)" in subsystem Methionine Biosynthesis (EC 2.3.1.46)

MetaCyc Pathways

aspartate superpathway (23/25 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
L-methionine biosynthesis I (4/5 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.46

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (312 amino acids)

>MIT1002_01112 Homoserine O-succinyltransferase (Alteromonas macleodii MIT1002)
MPIRIPEQLPAQNVLLGENIFTMDMDRAANQDIRPLEVGILNLMPNKIETEVQLLRLLSN
TPLQINVDLIRIDNQAPKNTPQSHMDAFYHDFSAVANKKYDGLIVTGAPLALIDYEEVKY
WETMTTILEWAQRHVNSTLYLCWAAHAAMYHFYGITRELRDEKFSGVFKHKVNDPNNELL
RGFDPSFYAPHSRYGHINTSLYNSVDGLSVVAESDDVGAYIVASEDKRMVFVTGHPEYDP
DTLKDEYFRDIAAGQTPVIPKNYFEGDDPAKSPIVQWRSHGSLLFTNWLNYYVYQTTPYD
LSQLAEKSQPKR