Protein Info for MIT1002_00948 in Alteromonas macleodii MIT1002

Annotation: 3-isopropylmalate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00180: Iso_dh
  • amino acids 6 to 357 (352 residues), 458.7 bits, E=7.6e-142
• TIGR00169: 3-isopropylmalate dehydrogenase
  • amino acids 6 to 357 (352 residues), 514.2 bits, E=8e-159

Best Hits

Swiss-Prot: 69% identical to LEU3_SHEON: 3-isopropylmalate dehydrogenase (leuB) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00052, 3-isopropylmalate dehydrogenase [EC: 1.1.1.85] (inferred from 98% identity to amc:MADE_03086)

MetaCyc: 68% identical to 3-isopropylmalate dehydrogenase (Escherichia coli K-12 substr. MG1655)
3-isopropylmalate dehydrogenase. [EC: 1.1.1.85]

Predicted SEED Role

"3-isopropylmalate dehydrogenase (EC 1.1.1.85)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 1.1.1.85)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• butanol and isobutanol biosynthesis (engineered) (2/8 steps found)
• aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.85

Protein Sequence (365 amino acids)

>MIT1002_00948 3-isopropylmalate dehydrogenase (Alteromonas macleodii MIT1002)
MSQYSIAVLAGDGIGPEVMQEANKVLDAVEKKFNVTLNRTAYPVGGYAIDTEGEALPSKT
LLGCEQADAILFGSIGGPKWDTLPLEQRPERAALLTLRSHFDLFSNLRPARIYPGLESLS
PLREDIAKSGVDVLVVRELTSGIYFGQPKGREGEGEEEFAYDTMRYSKREIRRIAIAAFE
AAQKRRGKVTSVDKANVLVTSRLWREIAEEVAKDYPDVELDHIYIDNATMQIMKNPAQFD
VMLCSNLFGDIVSDECAMMTGSMGLLPSASMNEDGFGLYEPAGGSAPDIAGQGIANPIAQ
ILSAAMMLRFSMNLNDAADAIEKAVVATLEAGVLTGELLPADKRSQAASTSQVGDEIVKQ
LMAQE