Protein Info for MIT1002_00905 in Alteromonas macleodii MIT1002

Annotation: Histidine ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 76% identical to HUTH_PSEA6: Histidine ammonia-lyase (hutH) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 91% identity to amc:MADE_03126)

MetaCyc: 67% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

L-histidine degradation VI (7/8 steps found)
L-histidine degradation I (4/4 steps found)
L-histidine degradation III (4/6 steps found)
L-histidine degradation II (3/5 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (527 amino acids)

>MIT1002_00905 Histidine ammonia-lyase (Alteromonas macleodii MIT1002)
MSHSSFFTENGGVKQLNLVPGTLTLDQLREVHRSHVHLSLDESAIPAINAAERVVLNVIK
ENRTVYGINTGFGLLANTRINVDELELLQRSIVLSHAAGTGDFMQEDTVRLLMLLKINSL
ARGYSGIRLSVIEALITLFNAQVYPAIPEKGSVGASGDLAPLAHMSVVLLGEGEAFYKGE
RISAAQALEIAGMQPIALAPKEGLALLNGTQASTAFALQGLFYTENALHSAIGIGALTVE
AALGSRVPFDARIHEVRGHKSQSDVATAFRTLLDTSEIGHSHQRCEKVQDPYSLRCQPQV
MGACLQQMRYAQEILVVEANGVSDNPLVFVENNDDDDTSTGDILSGGNFHAETVAMAADM
LAIALSEIGALSERRMSLMIDTHLSGLPPFLVENGGVNSGFMIAQVTCAALASENKTLAH
PASIDSLPTSANQEDHVSMATFAARRLRDIFDNVAGILAIEWLAACQGLDFRKPFKTSEK
LQVLMQLLRDQVPFYDKDRYFAPDIEKAKQLIKQHDLMDKTQLNLLG