Protein Info for MIT1002_00698 in Alteromonas macleodii MIT1002

Annotation: Nitrite reductase [NAD(P)H]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 849 PF07992: Pyr_redox_2" amino acids 9 to 296 (288 residues), 184 bits, see alignment E=2.2e-57 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 10 to 800 (791 residues), 1095.1 bits, see alignment E=0 PF13738: Pyr_redox_3" amino acids 73 to 281 (209 residues), 35.3 bits, see alignment E=3.9e-12 PF00070: Pyr_redox" amino acids 151 to 220 (70 residues), 57 bits, see alignment E=1.1e-18 PF18267: Rubredoxin_C" amino acids 321 to 386 (66 residues), 36.8 bits, see alignment 1.6e-12 PF04324: Fer2_BFD" amino acids 423 to 469 (47 residues), 38.1 bits, see alignment 7.9e-13 PF03460: NIR_SIR_ferr" amino acids 559 to 620 (62 residues), 47.1 bits, see alignment 8e-16 PF01077: NIR_SIR" amino acids 631 to 756 (126 residues), 79.5 bits, see alignment E=1e-25

Best Hits

Swiss-Prot: 59% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 97% identity to amc:MADE_03660)

MetaCyc: 59% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (849 amino acids)

>MIT1002_00698 Nitrite reductase [NAD(P)H] (Alteromonas macleodii MIT1002)
MTSLDSKTQIVVVGNGMVGHHFVEQLHQSDANVEINVLCGESRLAYDRVYLSSFFSGASA
DDLALTTPAQYAEWGVNVVTNALVTDIDRDNNKVTTSEGQVFSYDKLVLATGSYPFVPPI
PGNDQEHCLVYRTIDDLLAIEASAAVSKVGVVVGGGLLGLEAANALKQAGLDTHVVEFAP
QLMAVQLDQAGGNVLKDKIEALGVTVHTQKATQTIEAGDTCRYKMVFADGTALETDMILF
SAGIRPSDQLGRKSALTLGERGGIVIDNHCVTSDPNIYAVGECALWDNKIFGLVAPGYTM
ARTAVSHITGGDAEFAGADMSTKLKLMGVEVGSIGDAHERTVGALSYTYLNQPEGVYKKL
VVDSEQKYVLGAVLVGDTSDYDTLLQYALNNIELPEHPESLILPSSDAAPALGADALPDT
ASICSCLNVTKGDIVSAIEGGCCSVGDVKGETKASTGCGGCAALLKNVVDSELEKRGVEV
SKAICEHFNHTRQELFHIVKVNGIRTFDELLEQHGEGLGCEICKPAVGSILASVYNDYIL
KASHLPLQDTNDIYLGNMQKDGTYSVVPRVPGGEITPEKLILLGEVAKEYNLYTKITGGQ
RIDLFGARVEHLPDIWEKLVAGGFETGHAYAKALRTVKSCVGSTWCRYGVQDSVGTAIDL
ENRYKGLRAPHKIKFAVSGCTRECAEAQSKDIGVIATEQGWNLYVCGNGGMKPRHADLFA
TDLDTETLIKYIDRVLMFYVKTADRLQRTSVWMDNLEGGLAYLQDVVINDALGINSELEA
QMDAVVDAYQCEWKTTIENPESRKRFRQFVNSNASDTNIQFVSERGQVRPATEAEKVAGK
DQFIPVSMV