Protein Info for MIT1002_00495 in Alteromonas macleodii MIT1002

Annotation: Putative ribosome biogenesis GTPase RsgA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00157: ribosome small subunit-dependent GTPase A
  • amino acids 74 to 331 (258 residues), 288.1 bits, E=3e-90
• PF03193: RsgA_GTPase
  • amino acids 99 to 274 (176 residues), 199.9 bits, E=1.4e-63

Best Hits

Swiss-Prot: 57% identical to RSGA_SHEON: Small ribosomal subunit biogenesis GTPase RsgA (rsgA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K06949, ribosome biogenesis GTPase [EC: 3.6.1.-] (inferred from 95% identity to amc:MADE_00550)

MetaCyc: 63% identical to ribosome small subunit-dependent GTPase A (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Ribosome small subunit-stimulated GTPase EngC" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.1.15

Use Curated BLAST to search for 3.6.1.- or 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (347 amino acids)

>MIT1002_00495 Putative ribosome biogenesis GTPase RsgA (Alteromonas macleodii MIT1002)
MAKKPKLTQRQKRQVAANRSKRLREKHASKGAANLDESTLESGTVIGRFGKHADVEDTSG
AVSRCHIRRTVDSVVCGDKVFFSKGDDAESGVSGVIEIVKDRHSVLTRPDYYDGIKPIAA
NIDRIIVVSAILPILSLNIIDRYLVACEDIGITPLIVLNKVELLSEEERATVTEQLKTYE
NLGYEVLFTSCKTGEGISKLNEYLNDNISVFVGQSGVGKSSLINQVLPDADELTGDISDN
SGLGQHTTTAAKLLHLPAGGDLIDSPGVREFGLWHIPTERITWGFIEFRDYLGGCKFRDC
KHLNDPGCLLREAVEEGKISAERFDSYHRILTTMEEQRPSHSQPPGA