Protein Info for MIT1002_00368 in Alteromonas macleodii MIT1002

Annotation: 6'''-hydroxyparomomycin C oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 561 PF13450: NAD_binding_8" amino acids 10 to 48 (39 residues), 22.3 bits, see alignment 2e-08 PF00732: GMC_oxred_N" amino acids 82 to 334 (253 residues), 61 bits, see alignment E=2.2e-20 PF05199: GMC_oxred_C" amino acids 425 to 544 (120 residues), 79.8 bits, see alignment E=4.6e-26

Best Hits

KEGG orthology group: None (inferred from 100% identity to amc:MADE_00434)

MetaCyc: 74% identical to D-glucoside-3-dehydrogenase catalytic subunit (Agrobacterium fabrum)
GLUCOSIDE-3-DEHYDROGENASE-RXN [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]

Predicted SEED Role

"Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site" in subsystem Respiratory dehydrogenases 1

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.2.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (561 amino acids)

>MIT1002_00368 6'''-hydroxyparomomycin C oxidase (Alteromonas macleodii MIT1002)
MASNEYDAIVIGSGISGGWAAKELTEKGLKVLMLERGQNIEHIKDYKNAHKEDWDFPHRD
LPTQAMKVDYPVLKRDYPLNEKTYGMWANEKENPYTEDKRFDWYRGYHVGGRSLLWGRQS
YRLNKEDFEANAKEGIAVDWPIRYDDLAPWYDYVEKFAGISGNRDGLDVLPDGVYQPPMP
MNIVEESVAARVKKAYEGARHMVHGRTSNMTQSKPEEGRVHCQYRNKCWLGCPFGGYFST
QASTLPAAVKTGNLTLRPFSIVKEILFDKDKKRATGVEIIDAETNETYVFKSKIIFVNAS
ALNSAWVLMNSATDVWDGGLGSSSGELGHNVMDHHFRVGASAEVEGFDDKYTYGRRPSGF
YVPRFRNWGSDKRDYLRGFGYQGGASRSGWRKDIAELGIGAGLKDAITEPGPWTIGMTAF
GEILPYHENKVYLNKKVTDKWGLPVLAMDVEIKENELKMRKDMQQDAIEMFEAAGLKNVQ
GWDADYAPGMGIHEMGTARMGRDPKSSVLNAHNQVWDAPNVFVTDGAAMTSASCVNPSLT
YMALTARAADFAVEELKRGNL