Protein Info for MIT1002_00275 in Alteromonas macleodii MIT1002

Annotation: Hydrogenase-1 large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 625 PF00374: NiFeSe_Hases" amino acids 44 to 95 (52 residues), 73.7 bits, see alignment 7.8e-25 amino acids 132 to 610 (479 residues), 664.9 bits, see alignment E=4.9e-204

Best Hits

Swiss-Prot: 53% identical to MBHL_RHILV: Uptake hydrogenase large subunit (hupB) from Rhizobium leguminosarum bv. viciae

KEGG orthology group: K06281, hydrogenase large subunit [EC: 1.12.99.6] (inferred from 100% identity to amc:MADE_00347)

Predicted SEED Role

"Uptake hydrogenase large subunit (EC 1.12.99.6)" in subsystem Hydrogenases or Membrane-bound Ni, Fe-hydrogenase (EC 1.12.99.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.12.99.6

Use Curated BLAST to search for 1.12.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (625 amino acids)

>MIT1002_00275 Hydrogenase-1 large chain (Alteromonas macleodii MIT1002)
MENTASNNRLVVDPITRIEGHLRIEAEMDGNTIKQAFSSGTSVRGIELILQGRDPRDAWA
FAQRICGVCTLVHGMASVRAVEDAIRKAWRSNAKLGVAIGKPSMTSMPKGPMQHGKKGHR
QSRTSIGVLSEAEMAIPQNAQLIRNIMIATQYVHDHVMHFYHLHALDWVDVVSALDADPT
RTAALAGQLSDYPRSSPGYFKDMKQKVKTLVESGQLGIFSNAYWGHPGYKLPPEVNLMAL
AHYLDALTWQREVVKVHTIFGGKNPHPNFVVGGVPSPINLNASTGINTSRLVQLQDAITQ
MKSFVDQVYYPDIVAIAGYYKEWGTRGEGLGNFLTYGDLPMTSMDDPDSFLFPRGAILGR
DLSKVHDLDLDDPSEIQEFVSSSWYRYSGGNASGLHPFNGQTTLEYTGPKPPYKHLNVGA
EYSWLKSPRWKGHAMEVGPLARVLMMYAKKDAAAQDIVNRSLSILDLETSALFSTLGRTL
ARAVETKIVVNQLQSWYDQLLDNIAKGDTDTFNPLYFDPINWPIKGQGVGVMEAPRGALG
HWLVMQNGKIENYQCVVPTTWNAGPRDPNSQAGAYEAALQDKHTLHDPDQPLEILRTLHS
FDPCLACAVHVMDETGEERLRLKVR