Protein Info for MIT1002_00207 in Alteromonas macleodii MIT1002

Annotation: Pectic enzymes secretion protein OutD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 582 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF07655: Secretin_N_2" amino acids 154 to 263 (110 residues), 77.8 bits, see alignment E=7.6e-26 TIGR02519: pilus (MSHA type) biogenesis protein MshL" amino acids 258 to 560 (303 residues), 289.1 bits, see alignment E=1.5e-90 PF00263: Secretin" amino acids 384 to 561 (178 residues), 140.7 bits, see alignment E=3.6e-45

Best Hits

KEGG orthology group: K12282, MSHA biogenesis protein MshL (inferred from 96% identity to amc:MADE_00251)

Predicted SEED Role

"MSHA biogenesis protein MshL" in subsystem Mannose-sensitive hemagglutinin type 4 pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (582 amino acids)

>MIT1002_00207 Pectic enzymes secretion protein OutD (Alteromonas macleodii MIT1002)
MKLKNSLIVNTLVLSAALVVAGCQSYGVGEEVKPHIRDELARSQNTTSSKPTPPPQLNQE
LLAEVARYQALPAPTSQKMFAVSANNVDVRPFFDALVEDTPYSVAVHPAVSGQISLTLKE
VALEDVLTIISRMYPLDVFLEGKVIQVMPSQLKTESIPVNFLMIKRFGVSNVSVIAGGVS
EQDQNNGGNNGGINGNNNISGNALGGQGGFGGQGGLGGNNIQQLNGSNIQTTSENDFWSD
LKEALKVLVSPTEGRQVVVSPQAGLVTVRALPSEIAVVKDFLNQSQESLQRQVVLEARII
EVTLNDGYQQGVRWDRIASGLTGSVNFGFSGGAIASNFAANTAAGIDPAINAVNGIGNSI
STDIGGVSTLRISRGDFDGVINMLQTQGDVQMLSNPRVTVTNNQKAVIKVGQDEYFVTDV
STSEDQSATQTENENDIELTPFFSGIALDVTPQIDRSGSVILHVHPSVTETAEQTKVIQV
GDQQILLPLAQSNIRESDTVIRARDGEIVVIGGLMETVTSEQESKTPLLGDIPFIGNAFK
NKAKTQSKRELVILIRPSVVQPDTWEKQRLRTQSLLDQWYRN