Protein Info for MIT1002_00099 in Alteromonas macleodii MIT1002

Annotation: putative protoheme IX biogenesis protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 42 to 61 (20 residues), see Phobius details TIGR00540: heme biosynthesis-associated TPR protein" amino acids 7 to 385 (379 residues), 238 bits, see alignment E=7.3e-75 PF07219: HemY_N" amino acids 29 to 132 (104 residues), 78.1 bits, see alignment E=5.2e-26

Best Hits

KEGG orthology group: K02498, HemY protein (inferred from 94% identity to amc:MADE_00147)

Predicted SEED Role

"Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (391 amino acids)

>MIT1002_00099 putative protoheme IX biogenesis protein (Alteromonas macleodii MIT1002)
MKWLAIALAVLAAFVIALIVGPMLLGDKGYVLISLGNTAVEMTVISFCILVIGAVIAWYV
LSRLVLWALSLITGSHKWFGTLGERKRKRAFYDGLHAMAAGDFDTAQKALSKTTNGDFEG
VNYLASAQIAFANNDLAKARYFLVQATDFPKAKVAATVMHARIDMAEEKYDAALEKLNEL
DEQERENKQVVQLKAQILAKLGKWQVLQENLSGWRKALPKADYTTWSQRIAKGKFAEIAS
KQGAVELKSYWETLPRKLRHDDAYRAAYIQQLLDQGMHADAQNLLVEWQKRGPNSALFPL
FTQLNIPDASPSLRLLESWIKQDEENVSLYSTLGQVAFNSGDDVLAEKALLKATKMKSRK
EDLLLLSAISERKHDTATALQLYKEGQQLAS