Protein Info for MIT1002_00071 in Alteromonas macleodii MIT1002

Annotation: ATP-dependent DNA helicase rep

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 671 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01074: ATP-dependent DNA helicase Rep" amino acids 2 to 668 (667 residues), 1085.1 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 3 to 266 (264 residues), 306.3 bits, see alignment E=7e-95 PF13245: AAA_19" amino acids 7 to 249 (243 residues), 91.4 bits, see alignment E=1.7e-29 PF13361: UvrD_C" amino acids 271 to 614 (344 residues), 258.5 bits, see alignment E=3.9e-80 PF13538: UvrD_C_2" amino acids 555 to 609 (55 residues), 32.3 bits, see alignment 2.2e-11

Best Hits

Swiss-Prot: 67% identical to REP_ECOLI: ATP-dependent DNA helicase Rep (rep) from Escherichia coli (strain K12)

KEGG orthology group: K03656, ATP-dependent DNA helicase Rep [EC: 3.6.4.12] (inferred from 99% identity to amc:MADE_00076)

MetaCyc: 67% identical to ATP-dependent DNA helicase Rep (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase Rep" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (671 amino acids)

>MIT1002_00071 ATP-dependent DNA helicase rep (Alteromonas macleodii MIT1002)
MKLNEAQESAVTFVSGPCLVLAGAGSGKTRVITNKIAHLVRNCDMPARYIAAVTFTNKAA
REMKERVAQTLGKPEARGLKVSTFHTMGLTIIKAHVKDLGLKPGFSLFDDKDSFALLNDL
TSDTLDGDKDQLQLLQSCISNWKNDLILPDALLKSATSTGEREFAEAYKRYQDNLKAYNA
LDFDDLILLPTLLLKSSEAIRAKWQSKIRYLLVDEYQDTNTSQYELVKLLVGERARFTVV
GDDDQSIYSWRGAKPQNLHLLQQDFPRLNVIKLQQNYRSSGRILHCANILIQNNPHLFDK
TLFSELQYGEPLKVIEAKNEEHEGERVVAELLAHKFMNRTQFKDYAILYRGNHQSRIFEK
LLMSNRIPYKISGGMSFFGRAEVKDIMAYLRLLVNQDDDNALLRIINTPGRGIGRATLEK
LGNFANSLGVSMFEAATHPNLNSVLPDKAFHSVSTFARWIVELSDNAERGNTADAVRTMI
RTMGYEEWLYETSASPKAAEMAMANVSTLFGWVNDMLEGNDLDQPMTLTEVVNRLILRDM
MERGEDDGEGDQVQLMTLHASKGLEFPIVFLVGMEEGLLPHQSSVDEDNVEEERRLAYVG
ITRAQRELIFSLAKERRQFGEVINPEPSRFLFELPADDVQWENQKPKATKEERQQKAQVG
IANLRDILGKK