Protein Info for MIT1002_00007 in Alteromonas macleodii MIT1002

Annotation: Glycine--tRNA ligase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 301 TIGR00388: glycine--tRNA ligase, alpha subunit" amino acids 7 to 299 (293 residues), 567.9 bits, see alignment E=2.1e-175 PF02091: tRNA-synt_2e" amino acids 9 to 290 (282 residues), 505 bits, see alignment E=2.5e-156

Best Hits

Swiss-Prot: 99% identical to SYGA_ALTMD: Glycine--tRNA ligase alpha subunit (glyQ) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K01878, glycyl-tRNA synthetase alpha chain [EC: 6.1.1.14] (inferred from 99% identity to amc:MADE_00009)

MetaCyc: 82% identical to glycine--tRNA ligase subunit alpha (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (301 amino acids)

>MIT1002_00007 Glycine--tRNA ligase alpha subunit (Alteromonas macleodii MIT1002)
MQKYDIKTFQGLILALQDYWARQGCVIIQPLDMEVGAGTFHPMTFLRSLGPEPMSSAYVQ
PCRRPTDGRYGENPNRLQHYYQFQVMLKPSPDNIQELYLGSLKELGFDPLVHDIRFVEDN
WESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVTGEITYGLERLAMYVQGVDSI
YDLVWTDGPMGKVTYGDVFHQNEVEQSTYNFEHANVEALFRTFDECEAESKKLIENNLPL
PAYEQVMKASHAFNLLDARHAISVTERQRYILRVRTLAKACAESYYQAREALGFPLCNKE
A