Protein Info for MIT1002_00006 in Alteromonas macleodii MIT1002

Annotation: Glycine--tRNA ligase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 692 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 689 (689 residues), 880.6 bits, see alignment E=5.6e-269 PF02092: tRNA_synt_2f" amino acids 7 to 549 (543 residues), 695.7 bits, see alignment E=5.2e-213 PF05746: DALR_1" amino acids 580 to 681 (102 residues), 50.5 bits, see alignment E=2.1e-17

Best Hits

Swiss-Prot: 97% identical to SYGB_ALTMD: Glycine--tRNA ligase beta subunit (glyS) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 97% identity to amc:MADE_00008)

MetaCyc: 59% identical to glycine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (692 amino acids)

>MIT1002_00006 Glycine--tRNA ligase beta subunit (Alteromonas macleodii MIT1002)
MRTENCLIELGTEELPPKALKSLGEAFATQFEAALTQADLSFDSVSWFAAPRRLAVYVSG
LAEGQADKVVEKRGPAVSAAFDADGNPTKAAQGWARGNGIDVADAERLVTDKGEWLLHKA
HVPGQLVAELLEGLINQAVSKLPIPKPMRWGNYNTQFIRPVHTLCVLYGSEVVNVSVLGL
TSGRVVQGHRFHGEGRFELDHADNYASALEQQYVLADFEARKDKVRQQLEDAAQSLSLKP
DYNEDLLEEIASLVEWPVVLQAGFDEAFLAVPKEALIYTMKDDQKYVPLLDSDGALSNTF
LFVTNIESRDASQVISGNEKVIRPRLADAEFFFNSDKKTTLESRLESLETVLFQKQLGTL
KEKSERISALSAFIASQIDANETQAARAGLLAKTDLMSNMVMEFPDVQGVMGKYYALNDG
EDAPVAEALYEQYMPRFAGDALPSSGVSASVALADKLDTLVGIFGIGQLPKGDKDPFALR
RAAIGVLRIVTELSLPLDLETLVSKAIDVYGDKLTNAETQSQVVDFVLGRFTALLQDQAI
AIDVIQAVAARRPTKPADYLARVHAVDKFRALEEAEALAAANKRVANILAKQNVEVTDTV
NIDESLLAEEAEKALYVELKAAQKEVDIAVPSQDYTRILTALATLRNVIDNFFDNVMVMA
DDEAVKNNRLALLSLLRQLFLTTADISILAKS