Protein Info for MCAODC_25315 in Escherichia coli ECRC101

Name: gsiA
Annotation: glutathione ABC transporter ATP-binding protein GsiA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 623 PF00005: ABC_tran" amino acids 33 to 197 (165 residues), 106.6 bits, see alignment E=6.7e-34 amino acids 341 to 491 (151 residues), 111.4 bits, see alignment E=2.3e-35 PF08352: oligo_HPY" amino acids 248 to 280 (33 residues), 33.6 bits, see alignment (E = 1.7e-11) amino acids 543 to 575 (33 residues), 34.1 bits, see alignment (E = 1.2e-11) PF13401: AAA_22" amino acids 353 to 519 (167 residues), 27.2 bits, see alignment E=1.7e-09

Best Hits

Swiss-Prot: 100% identical to GSIA_ECO57: Glutathione import ATP-binding protein GsiA (gsiA) from Escherichia coli O157:H7

KEGG orthology group: K13892, glutathione transport system ATP-binding protein (inferred from 100% identity to ecf:ECH74115_0980)

MetaCyc: 99% identical to glutathione ABC transporter ATP binding subunit GsiA (Escherichia coli K-12 substr. MG1655)
RXN0-11 [EC: 7.4.2.10]

Predicted SEED Role

"Dipeptide transport ATP-binding protein DppD in protein degradation cluster"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (623 amino acids)

>MCAODC_25315 glutathione ABC transporter ATP-binding protein GsiA (Escherichia coli ECRC101)
LPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGETLAIVGESGSGKSVTAL
ALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVRGADMAMIFQEPMTSLNPV
FTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQTILSRYPHQLSGGMRQRVMIA
MALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEMSMGVIFITHDMGVVAEIADRVL
VMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLGAMKGLDYPRRFPLISLEHPAKQAP
PIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVTREVHAVEKVSFDLWPGETLSLVGESG
SGKSTTGRALLRLVESQGGEIIFNGQRIDTLSPGKLQALRRDIQFIFQDPYASLDPRQTI
GDSIIEPLRVHGLLPGKEAVARVAWLLERVGLLPEHAWRYPHEFSGGQRQRICIARALAL
NPKVIIADEAVSALDVSIRGQIINLLLDLQRDFGIAYLFISHDMAVVERISHRVAVMYLG
QIVEIGPRRAVFENPQHPYTRKLLAAVPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAV
SLQCVGPGHYVAQPQSEYAFMRR