Protein Info for MCAODC_24025 in Escherichia coli ECRC101

Name: rutF
Annotation: pyrimidine utilization flavin reductase protein F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 164 TIGR03615: pyrimidine utilization flavin reductase protein F" amino acids 4 to 159 (156 residues), 288.8 bits, see alignment E=5.2e-91 PF01613: Flavin_Reduct" amino acids 13 to 159 (147 residues), 134.7 bits, see alignment E=1.5e-43

Best Hits

Swiss-Prot: 100% identical to RUTF_ECO5E: FMN reductase (NADH) RutF (rutF) from Escherichia coli O157:H7 (strain EC4115 / EHEC)

KEGG orthology group: K09024, putative flavin reductase RutF [EC: 1.5.1.-] (inferred from 98% identity to eco:b1007)

MetaCyc: 98% identical to FMN reductase RutF (Escherichia coli K-12 substr. MG1655)
RXN-9510 [EC: 1.5.1.42]

Predicted SEED Role

"Predicted flavin reductase RutF in novel pyrimidine catabolism pathway" in subsystem Pyrimidine utilization

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.-

Use Curated BLAST to search for 1.5.1.- or 1.5.1.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (164 amino acids)

>MCAODC_24025 pyrimidine utilization flavin reductase protein F (Escherichia coli ECRC101)
MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVW
PVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQNGVTGCPQLEEALVSFDCR
ISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALMRPAC