Protein Info for MCAODC_15850 in Escherichia coli ECRC101

Name: puuE
Annotation: 4-aminobutyrate transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00700: 4-aminobutyrate transaminase
  • amino acids 8 to 421 (414 residues), 753 bits, E=3.5e-231
• PF00202: Aminotran_3
  • amino acids 21 to 419 (399 residues), 439.3 bits, E=1.2e-135

Best Hits

Swiss-Prot: 99% identical to PUUE_ECOLI: 4-aminobutyrate aminotransferase PuuE (puuE) from Escherichia coli (strain K12)

KEGG orthology group: K00823, 4-aminobutyrate aminotransferase [EC: 2.6.1.19] (inferred from 100% identity to etw:ECSP_1827)

MetaCyc: 99% identical to 4-aminobutyrate aminotransferase PuuE (Escherichia coli K-12 substr. MG1655)
5-aminovalerate transaminase. [EC: 2.6.1.48]; 4-aminobutyrate transaminase. [EC: 2.6.1.48, 2.6.1.19]

Predicted SEED Role

"Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)" (EC 2.6.1.19)

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (13/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (11/11 steps found)
• L-lysine degradation I (7/7 steps found)
• superpathway of 4-aminobutanoate degradation (3/3 steps found)
• L-lysine degradation X (5/6 steps found)
• 4-aminobutanoate degradation I (2/2 steps found)
• 4-aminobutanoate degradation II (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• GABA shunt I (3/4 steps found)
• GABA shunt II (3/4 steps found)
• L-lysine degradation IV (2/5 steps found)
• L-lysine degradation III (2/6 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)
• nicotine degradation I (pyridine pathway) (6/17 steps found)
• superpathway of L-lysine degradation (14/43 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Butanoate metabolism
• Glutamate metabolism
• Lysine degradation
• Propanoate metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.19

Use Curated BLAST to search for 2.6.1.19 or 2.6.1.48

Protein Sequence (421 amino acids)

>MCAODC_15850 4-aminobutyrate transaminase (Escherichia coli ECRC101)
MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPD
LVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKI
ARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHGISTQ
DSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
QSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGN
PLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTLIDAKENVPAIAAVRGLGSMIAAEFND
PQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDALR
D