Protein Info for MCAODC_15745 in Escherichia coli ECRC101

Name: pdeR
Annotation: cyclic di-GMP phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 525 PF00989: PAS" amino acids 114 to 169 (56 residues), 28.8 bits, see alignment 2.1e-10 TIGR00229: PAS domain S-box protein" amino acids 115 to 233 (119 residues), 47.7 bits, see alignment E=1.6e-16 PF13426: PAS_9" amino acids 123 to 225 (103 residues), 36.2 bits, see alignment E=1.3e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 235 to 394 (160 residues), 120.7 bits, see alignment E=5.2e-39 PF00990: GGDEF" amino acids 237 to 390 (154 residues), 158.9 bits, see alignment E=1.9e-50 PF00563: EAL" amino acids 412 to 517 (106 residues), 94.9 bits, see alignment E=1e-30

Best Hits

KEGG orthology group: K14051, cyclic di-GMP phosphodiesterase Gmr [EC: 3.1.4.52] (inferred from 100% identity to etw:ECSP_1806)

Predicted SEED Role

"FIG00637999: hypothetical protein"

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.52

Use Curated BLAST to search for 3.1.4.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (525 amino acids)

>MCAODC_15745 cyclic di-GMP phosphodiesterase (Escherichia coli ECRC101)
MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTLQLSAEQAARIREMTVI
TSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAVARDLSHGLSFAEQVVSEA
HSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLFMSRREAAASRRNNRVFFRSG
NAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLICSGTDITEERRAQERLRILANTD
SITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDNFKKVNDAYGHLFGDQLLRDVSLAI
LSCLEHDQVLARPGGDEFLVLASNTSQSALEAMASRILTRLRLPFRIGLIEVYTSCSVGI
ALSPEHGSDSAAIIRHADTAMYTAKEGGRGQFCVFTPEMNQRVFEYLWLDTNLRKALEND
QLVIHYQPKITWRGEVRSLEALVRWQSPERGLIPPLDFISYAEESGLIVPLGRWVILDVV
RQVAKWRDKGINLRVAVNISARQLADQTIFTALKQVLQELNFEYY