Protein Info for LU632_RS22480 in Erwinia tracheiphila SCR3

Name: rsmB
Annotation: 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01029: NusB
  • amino acids 6 to 127 (122 residues), 89.4 bits, E=7.1e-29
• TIGR00563: 16S rRNA (cytosine(967)-C(5))-methyltransferase
  • amino acids 8 to 428 (421 residues), 555.2 bits, E=6.3e-171
• PF01189: Methyltr_RsmB-F
  • amino acids 240 to 426 (187 residues), 241.6 bits, E=1.5e-75
• PF01728: FtsJ
  • amino acids 247 to 370 (124 residues), 25.1 bits, E=3.8e-09
• PF13847: Methyltransf_31
  • amino acids 247 to 385 (139 residues), 32.5 bits, E=1.7e-11
• PF13649: Methyltransf_25
  • amino acids 250 to 320 (71 residues), 27 bits, E=1.5e-09

Best Hits

Swiss-Prot: 78% identical to RSMB_ERWT9: Ribosomal RNA small subunit methyltransferase B (rsmB) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K03500, ribosomal RNA small subunit methyltransferase B [EC: 2.1.1.-] (inferred from 79% identity to ebi:EbC_40990)

MetaCyc: 70% identical to 16S rRNA m5C967 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11591 [EC: 2.1.1.176]

Predicted SEED Role

"Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase (EC 2.1.1.-)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.176

Protein Sequence (429 amino acids)

>LU632_RS22480 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB (Erwinia tracheiphila SCR3)
MKKMTNLRALAAQTMEKVVEQGLSLSNVLPAAQRNRSEKDQALLQELSYGVLRTLPQLEW
LISQLMERPMTGKQRSIHFLIMTGLYQLIFTRIPAHAALAETVEGAVALKRPQLKGLING
VLRQFQRKQEDLMLTMSKVEQNYLHPKWLLDRLKQAWPEQWRDIVDANNQRPPMWLRVNR
QHHTRDAWLELLKEQGKSAEPHPVHPDALRLQSPCPVNQLPGFEKGWVSVQDASAQGCVA
LLSPQNHEAILDLCAAPGGKTTHILEAAPLARVTAVDIDAQRLARVRENLQRLNMSADVK
SGDATTPEKWCGQTRFDRILLDAPCSATGVIRRHPDIKWLRRDRDISELAALQQQILDAI
WPYLKPGGILLYATCSILPEENHLQIAAFLARNIDAQLKETGNTEHPGLQIFPEPAGGDG
FYYAKLTKI