Protein Info for LU632_RS21845 in Erwinia tracheiphila SCR3

Name: fieF
Annotation: CDF family cation-efflux transporter FieF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 39 to 60 (22 residues), see Phobius details amino acids 81 to 102 (22 residues), see Phobius details amino acids 114 to 136 (23 residues), see Phobius details amino acids 158 to 175 (18 residues), see Phobius details amino acids 181 to 199 (19 residues), see Phobius details TIGR01297: cation diffusion facilitator family transporter" amino acids 12 to 288 (277 residues), 242.3 bits, see alignment E=3.1e-76 PF01545: Cation_efflux" amino acids 15 to 206 (192 residues), 158.7 bits, see alignment E=1.6e-50 PF16916: ZT_dimer" amino acids 210 to 287 (78 residues), 88.1 bits, see alignment E=3.4e-29

Best Hits

Swiss-Prot: 78% identical to FIEF_CROS8: Cation-efflux pump FieF (fieF) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: K13283, ferrous-iron efflux pump FieF (inferred from 80% identity to ebi:EbC_01300)

MetaCyc: 75% identical to Zn2+/Fe2+/Cd2+ exporter (Escherichia coli K-12 substr. MG1655)
RXN0-6; TRANS-RXN0-200; TRANS-RXN0-244

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (297 amino acids)

>LU632_RS21845 CDF family cation-efflux transporter FieF (Erwinia tracheiphila SCR3)
MEQHYARRVNSATLAATTLASVLLLIKVFAWWYTGSVSLLAALVDSLVDVAASLTNLLVV
RYSLQPADDEHTFGHGKAESLAALAQSMFISGSAFFLFLTGIQHFATPMEMRAPLIGVMV
TLIALFSTLILVTFQRWVVRHTHSQAVRADMLHYQSDVIMNGAILVALVLSWYGFRRADA
FFALGIGVWILYSALRMGYDAVQSLLDRALPEDDRREINAIVSSWPGVRGVHDIRTRQSG
PTRFIQLHLELDDDLPLLYAHRIATEVELALLAGFPGADITIHQDPCSVVPEQHHMR