Protein Info for LU632_RS09965 in Erwinia tracheiphila SCR3

Name: rlmKL
Annotation: bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 705 PF02926: THUMP" amino acids 22 to 153 (132 residues), 70.6 bits, see alignment E=6.3e-23 PF01170: UPF0020" amino acids 162 to 375 (214 residues), 212 bits, see alignment E=2.8e-66 PF10672: Methyltrans_SAM" amino acids 474 to 658 (185 residues), 83.5 bits, see alignment E=6.3e-27 PF03602: Cons_hypoth95" amino acids 532 to 638 (107 residues), 34.2 bits, see alignment E=8.2e-12

Best Hits

Swiss-Prot: 82% identical to RLMKL_ERWT9: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 84% identity to ebi:EbC_15330)

MetaCyc: 77% identical to fused 23S rRNA m2G2445 methyltransferase and 23S rRNA m7G2069 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6950 [EC: 2.1.1.264]; RXN-11574 [EC: 2.1.1.264, 2.1.1.173]

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173 or 2.1.1.264

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (705 amino acids)

>LU632_RS09965 bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL (Erwinia tracheiphila SCR3)
MNSLFASTARGLEELLKSELEALGASDCQVVQGGVHYKGDDRLMYQSLLWSRLASRILLP
LTECGIYSDLDLYLGVQAVNWPAMFGSDKTFAVHFSGLNEVIRNSQYGALKVKDAIVDSF
TRKNLPRPDVDREQPDIRINVWLYNDKASIALDLSGDGLHQRGYRQQTGQAPLKENLAAA
VVLRSGWKPGSTMVDPMCGSGTLLIEAAMIASDRAPGLHRNHWGFNGWSQFNATLWRELK
SEAQARASRGLRATTSRFYGYDNDPKVIERARVNARKAGLADLVGFTAQDVLKLTNPQQE
GSVGTLLSNPPYGERLESKPALIALYSQLGRLMKQHFAGWNLSLFSASPELLSCLQLRAE
RQFKAKNGPLDCVQKNYQLAASTMSTGQAKIAEDYANRLRKNLKKLGKWASQSGIECYRL
YDADLPEYNVAVDRYADWVVVQEYAPPKTIDAAKARQRLFDVIAATLSVLELPANRLVLK
TRERQKGKSQYQKLGEKGDFFEVAEFNAKLWVNLTDYLDTGLFLDHRIARKMLGEMSRGK
DFLNLFAYTGTASVHAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTGRQHRLIQADCLS
WLNESDEIYDLIFIDPPTFSNSKRMEESFDVQRDHLLLLKNLHRLLRKGGTIMFSNNKRG
FKMDHDGLRELHFSAQDITVGTQSQDFARNRQIHNCWLITHAGKE