Protein Info for LU632_RS08255 in Erwinia tracheiphila SCR3

Name: ppx
Annotation: exopolyphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 510 transmembrane" amino acids 270 to 288 (19 residues), see Phobius details amino acids 433 to 446 (14 residues), see Phobius details TIGR03706: exopolyphosphatase" amino acids 14 to 307 (294 residues), 323.7 bits, see alignment E=5.8e-101 PF02541: Ppx-GppA" amino acids 27 to 309 (283 residues), 343.1 bits, see alignment E=1.4e-106 PF21447: Ppx-GppA_III" amino acids 315 to 491 (177 residues), 206.5 bits, see alignment E=2.8e-65

Best Hits

Swiss-Prot: 78% identical to PPX_SALTI: Exopolyphosphatase (ppx) from Salmonella typhi

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 88% identity to ebi:EbC_33600)

MetaCyc: 78% identical to exopolyphosphatase (Escherichia coli K-12 substr. MG1655)
Exopolyphosphatase. [EC: 3.6.1.11]

Predicted SEED Role

"Exopolyphosphatase (EC 3.6.1.11)" in subsystem Phosphate metabolism (EC 3.6.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.11, 3.6.1.40

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (510 amino acids)

>LU632_RS08255 exopolyphosphatase (Erwinia tracheiphila SCR3)
MPISHKKMPKPQEFAAIDLGSNSFHMVIARVVDGAMQVLGRLKQRVHLADGLDNNNILSE
DAIQRGLACLTLFAERLQGFSPANVTIVGTHTLRQAMNAEDFLQRAAEVIPYPIEVISGH
EEARLIFMGVEHTQPEKGRKLVIDIGGGSTEMVIGEDFEPRLVESRRMGCVSFANLYFPG
GTISKKNFRRARLATRQKLETLAWQYRLMGWQYALGASGTIKAACKVLQAMGEKEKLITP
ERLDKLYDETIRYKSFGSLSLPGLSDERKAVFVPGLAILCGVFDALAIRELRLSNGALRE
GVLYEMEGRFRHQDIRSRTAQSLANHYAIDSDQAGRVLETTKQLYVQWRDQNPRQANPQL
SALLKWAAMLHEVGLTINHSGLQRHSSYILQNTNLPGFNQDQQTLLATLVRFHRKAVKVD
EMPRLTLFKKKQFLPLIFLLRLGALLNNQRQATTTPSSLKLETDDGHWTLTFPHDYFSQN
NLVQLDLEREQSYWKDATGWKLMIQEEPGE