Protein Info for LU632_RS06405 in Erwinia tracheiphila SCR3

Name: cysI
Annotation: assimilatory sulfite reductase (NADPH) hemoprotein subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02041: sulfite reductase (NADPH) hemoprotein, beta-component
  • amino acids 20 to 559 (540 residues), 958.4 bits, E=5e-293
• PF03460: NIR_SIR_ferr
  • amino acids 81 to 141 (61 residues), 50.1 bits, E=1.8e-17
  • amino acids 355 to 415 (61 residues), 39.1 bits, E=5.1e-14
• PF01077: NIR_SIR
  • amino acids 174 to 329 (156 residues), 148.1 bits, E=1.5e-47

Best Hits

Swiss-Prot: 87% identical to CYSI_ERWT9: Sulfite reductase [NADPH] hemoprotein beta-component (cysI) from Erwinia tasmaniensis (strain DSM 17950 / CIP 109463 / Et1/99)

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 90% identity to ebi:EbC_35380)

MetaCyc: 82% identical to sulfite reductase, hemoprotein subunit (Escherichia coli K-12 substr. MG1655)
Sulfite reductase (NADPH). [EC: 1.8.1.2]

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (9/12 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (6/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Protein Sequence (571 amino acids)

>LU632_RS06405 assimilatory sulfite reductase (NADPH) hemoprotein subunit (Erwinia tracheiphila SCR3)
MSNEKHPGPLVVEGKLTDAERLKKQSNYLRGTLTDDLQDGLTGGFSGDNFLLIRFHGMYQ
QDDRDIRAERAEQKLEPRHAMMLRCRLPGGIITPTQWLTIDKFAEENTLYGSIRLTNRQT
FQFHGILKGNVKPAHQMLNEVGLDALATANDVNRNVLCTSNPLESELHQEAYEWAKKISE
HLLPQTRAYAEIWLDREKVATTDNEPILGETYLPRKFKTTVVVPPHNDVDLHANDLNFIA
IAEEGKLIGFNLLVGGGLSIDHGNKATYARTASEFGYLPLDKTLAVAEAVVTTQRDWGNR
TDRKNAKTKYTLERVGVETFKAEVERRAGLTFEPVRPYEFTTRGDHFGWVKGIDNQWHLT
LFIENGRLLDYPGRPLKSGVAEIARVHTGDFRLTANQNLIVAGVSAANKAQIEQLARDHG
LMEAVSTQRENSMACVSFPTCPLAMAEAERFLPEFVTRVESIMSAHGVGDEHIVLRVTGC
PNGCGRALLAEIGLVGKAPGRYNLHLGGNRIGTRIPRMYRENISEEEILSTLDQLVGRWS
AEREVGEGFGDFAVRVGIVKAVVDPARDFWQ