Protein Info for LU632_RS05915 in Erwinia tracheiphila SCR3

Name: gcvP
Annotation: aminomethyl-transferring glycine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02347: GDC-P
  • amino acids 16 to 440 (425 residues), 608.8 bits, E=5.5e-187
  • amino acids 463 to 739 (277 residues), 32.5 bits, E=4.6e-12
• TIGR00461: glycine dehydrogenase
  • amino acids 17 to 950 (934 residues), 1667.9 bits, E=0
• PF21478: GcvP2_C
  • amino acids 781 to 902 (122 residues), 192.6 bits, E=1.9e-61

Best Hits

Swiss-Prot: 84% identical to GCSP_CITK8: Glycine dehydrogenase (decarboxylating) (gcvP) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 68% identity to avr:B565_1479)

MetaCyc: 83% identical to glycine decarboxylase (Escherichia coli K-12 substr. MG1655)
Glycine dehydrogenase (decarboxylating). [EC: 1.4.1.27, 1.4.4.2]

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

• folate transformations III (E. coli) (8/9 steps found)
• folate transformations II (plants) (9/11 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• glycine degradation (3/3 steps found)
• photorespiration II (6/10 steps found)
• folate transformations I (8/13 steps found)
• photorespiration I (5/9 steps found)
• photorespiration III (5/9 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.27 or 1.4.4.2

Protein Sequence (960 amino acids)

>LU632_RS05915 aminomethyl-transferring glycine dehydrogenase (Erwinia tracheiphila SCR3)
MTQTLRQLEHTGAFIERHIGPSPAQQDAMLSAIGARSLSDLIASIVPADIQLPGPPAIGD
ALTEHQALTELKAIASQNQRYKSYIGMGYTPVLTPPVILRNMLENPGWYTAYTPYQPEIS
QGRLEALLNFQQITLDLTGLDMASASLLDEATAAAEAMAMAKRISKLKNAHRFFVADDIH
PQTLDVVRTRAEPFGFEVITDKAEKVLDYQDVFGVLLQQVGTGGEVHHYASLIATLKARK
VIVAVAADFMSLVLLEAPGKQGADIVFGSAQRFGVPMGYGGPHAAFFAARDEHKRSMPGR
IIGVSRDSAGNTALRMAMQTREQHIRREKANSNICTSQVLLANIASLYAVLHGPAGLKRI
ASRIHRLTDILAAGLQKGGLTLRHQSWFDTLTVNVTDKAAVLDRALSFGVNLRTDIYHAV
GITLDETTQREDIAVLFSILLGDNHGLDIDQLDADAVENSDSVPAGMLREDAILTHPVFN
RHHSETEMMRYMHGLERKDLALNQAMIPLGSCTMKLNAAAEMIPITWPEFSELHPFCPAE
QAGGYLQMIGQLSQWLVQLTGYDALCMQPNSGAQGEYAGLLAIRRYHESRNESGRHICLI
PSSAHGTNPASAQMAGMAVVVVACDKQGNIDLHDLREKAEKAGEALSCIMVTYPSTHGVY
EETIREVCQIVHQYGGQVYLDGANMNAQVGITTPGYIGADVSHLNLHKTFCIPHGGGGPG
MGPVGVKAHLAPFVPGHSVVQIDGVLTQQGAVCAAPFGSASILPISWMYIRMMGAEGLKQ
ASSVAILNANYIARRLQSAWPVLYAGLDGRVAHECILDIRPLKEQTGISELDIAKRLIDY
GFHAPTMSFPVAGTLMVEPTESESKIELDRFIDAMLSIRMEIDRVAAGEWPVDDNPLVNA
PHTQMEMVGEWSHPYTRELAVFPAGSDNKYWPTVKRLDDVYGDRNLFCSCVPMSEYEQFC