Protein Info for LU632_RS02415 in Erwinia tracheiphila SCR3

Name: mqo
Annotation: malate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 30 to 517 (488 residues), 758.9 bits, E=3.1e-232
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 31 to 515 (485 residues), 662.5 bits, E=1.8e-203
• PF01266: DAO
  • amino acids 32 to 304 (273 residues), 38.3 bits, E=1.8e-13

Best Hits

Swiss-Prot: 83% identical to MQO_CROS8: Probable malate:quinone oxidoreductase (mqo) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 83% identity to esa:ESA_03816)

MetaCyc: 69% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.4

Use Curated BLAST to search for 1.1.5.4

Protein Sequence (550 amino acids)

>LU632_RS02415 malate dehydrogenase (quinone) (Erwinia tracheiphila SCR3)
MNTLSKISQVISLATLLACSSTYAENTAEKTDVLLIGGGIMSASLGTWLQELQPDWKQLM
VEKLDGVALESSNGWSNAGTGHSANMELNYTPERQDGSINFSKALEINELFMISRQFWTS
QVQRGVLQDPHSFINSTPHMSFVWGDKNVDYLTRRYKALQQTTLFQGMQFSTDHKQIAQW
APLIMEGRDPQQKVAATWTPVGTDVNYGEITRQLIGSLKKHDNFKLETSTEVTGFKRNSD
NSWHVTVTDLKDGKEHEVDAKYVFIGAGGGALKLLQKTGIPEADNYAGFPVGGSFLMTEN
PQITSQHLEKVYGQASVGAPPMSVPHLDARFLDGKRVVLFGPFATFSTKFLKNGSYMDLL
KTTTTSNFMPMMHVGMDNFDLVKYLIGQVMLNDDDRFAALKEYYPAARKEDWKLIQAGQR
VQIIKKDADKGGLLKLGTEVVTDQQKTVAALLGASPGASTAAPITLNVIKKLFPEQFNSS
EWQSKIKAIILSFGQELNGNVALTQQVWDNTAATLQLTKPPVIQMNSTSPANAQAEPQKA
AAKDHHYMAL